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DC Field | Value | Language |
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dc.contributor.author | Valero Gils, Rebeca | cat |
dc.contributor.author | Bayés Colomer, Mònica | cat |
dc.contributor.author | Sánchez-Font, Ma. Francisca | cat |
dc.contributor.author | Gonzàlez-Angulo, Olga | cat |
dc.contributor.author | Gonzàlez-Duarte, Roser | cat |
dc.contributor.author | Marfany i Nadal, Gemma | cat |
dc.date.accessioned | 2009-03-26T08:49:54Z | - |
dc.date.available | 2009-03-26T08:49:54Z | - |
dc.date.issued | 2001 | cat |
dc.identifier.issn | 1465-6914 | cat |
dc.identifier.uri | http://hdl.handle.net/2445/7381 | - |
dc.description.abstract | Background: The ubiquitin-dependent protein degradation pathway is essential for the proteolysis of intracellular proteins and peptides. Deubiquitinating enzymes constitute a complex protein family involved in a multitude of cellular processes. The ubiquitin-specific proteases (UBP) are a group of enzymes whose predicted function is to reverse the ubiquitinating reaction by removing ubiquitin from a large variety of substrates. We have lately reported the characterization of human USP25, a specific-ubiquitin protease gene at 21q11.2, with a specific pattern of expression in murine fetal brains and adult testis. Results: Database homology searches at the DNA and protein levels and cDNA library screenings led to the identification of a new UBP member in the human genome, named USP28, at 11q23. This novel gene showed preferential expression in heart and muscle. Moreover, cDNA, expressed sequence tag and RT-PCR analyses provided evidence for alternatively spliced products and tissue-specific isoforms. Concerning function, USP25 overexpression in Down syndrome fetal brains was shown by real-time PCR. Conclusions: On the basis of the genomic and protein sequence as well as the functional data, USP28 and USP25 establish a new subfamily of deubiquitinating enzymes. Both genes have alternatively spliced exons that could generate protein isoforms with distinct tissue-specific activity. The overexpression of USP25 in Down syndrome fetal brains supports the gene-dosage effects suggested for other UBP members related to aneuploidy syndromes. | eng |
dc.format.extent | 10 p. | cat |
dc.format.mimetype | application/pdf | eng |
dc.language.iso | eng | eng |
dc.publisher | BioMed Central | cat |
dc.relation.isformatof | Reproducció del document publicat a http://dx.doi.org/10.1186/gb-2001-2-10-research0043 | cat |
dc.relation.ispartof | Genome Biology, 2001, vol. 2, núm. 10 | cat |
dc.relation.uri | http://dx.doi.org/10.1186/gb-2001-2-10-research0043 | - |
dc.rights | cc-by, (c) Valero et al., 2001 | cat |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0/ | cat |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Biologia cel·lular | cat |
dc.subject.classification | Genètica molecular | cat |
dc.subject.classification | Medicina | cat |
dc.subject.classification | Neurobiologia | cat |
dc.subject.other | Cell biology | eng |
dc.subject.other | Molecular biology | eng |
dc.subject.other | Medicine | eng |
dc.subject.other | Neurobiology | eng |
dc.title | Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25 | eng |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 520442 | cat |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 11597335 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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520442.pdf | 461.72 kB | Adobe PDF | View/Open |
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