Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/8302
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yi, Jian-Ri | cat |
dc.contributor.author | Lu, Shilun | cat |
dc.contributor.author | Fernández-Checa Torres, José Carlos | cat |
dc.contributor.author | Kaplowitz, Neil | cat |
dc.date.accessioned | 2009-05-15T08:16:10Z | - |
dc.date.available | 2009-05-15T08:16:10Z | - |
dc.date.issued | 1994 | cat |
dc.identifier.issn | 0021-9738 | cat |
dc.identifier.uri | http://hdl.handle.net/2445/8302 | - |
dc.description.abstract | Using the Xenopus oocyte expression system, we have previously identified an approximately 4-kb fraction of mRNA from rat liver that expresses sulfobromophthalein-glutathione (BSP-GSH)-insensitive reduced glutathione (GSH) transport (Fernandez-Checa, J., J. R. Yi, C. Garcia-Ruiz, Z. Knezic, S. Tahara, and N. Kaplowitz. 1993. J. Biol. Chem. 268:2324-2328). Starting with a cDNA library constructed from this fraction, we have now isolated a single clone that expresses GSH transporter activity. The cDNA for the rat canalicular GSH transporter (RcGshT) is 4.05 kb with an open reading frame of 2,505 nucleotides encoding for a polypeptide of 835 amino acids (95,785 daltons). No identifiable homologies were found in searching various databases. An approximately 96-kD protein is generated in in vitro translation of cRNA for RcGshT. Northern blot analysis reveals a single 4-kb transcript in liver, kidney, intestine, lung, and brain. The abundance of mRNA for RcGshT in rat liver increased 3, 6, and 12 h after a single dose of phenobarbital. Insensitivity to BSP-GSH and induction by phenobarbital, unique characteristics of canalicular GSH secretion, suggest that RcGshT encodes for the canalicular GSH transporter. | eng |
dc.format.extent | 5 p. | cat |
dc.format.mimetype | application/pdf | eng |
dc.language.iso | eng | eng |
dc.publisher | American Society for Clinical Investigation | cat |
dc.relation.isformatof | Reproducció del document publicat a http://dx.doi.org/10.1172/JCI117170 | cat |
dc.relation.ispartof | Journal of Clinical Investigation, 1994, vol. 93, núm. 4, p. 1841-1845. | cat |
dc.relation.uri | http://dx.doi.org/10.1172/JCI117170 | - |
dc.rights | (c) The American Society for Clinical Investigation, 1994 | cat |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | Glutatió | cat |
dc.subject.classification | Fetge | cat |
dc.subject.classification | Metabolisme | cat |
dc.subject.classification | Proteïnes de membrana | cat |
dc.subject.classification | Transport biològic | cat |
dc.subject.classification | Clonatge | cat |
dc.subject.other | Glutathione | eng |
dc.subject.other | Liver | eng |
dc.subject.other | Membrane proteins | eng |
dc.subject.other | Metabolism | eng |
dc.subject.other | Cloning | eng |
dc.title | Expression cloning of a rat hepatic reduced glutathione transporter with canalicular characteristics | eng |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 89661 | cat |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 8163683 | - |
Appears in Collections: | Articles publicats en revistes (Medicina) |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.