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Title: Prostate tumor OVerexpressed-1 (PTOV1) down-regulates HES1 and HEY1 notch targets genes and promotes prostate cancer progression
Author: Alaña, Lide
Sesé, Marta
Cánovas, Verónica
Punyal, Yolanda
Fernández, Yolanda
Abasolo, Ibane
Torres, Inés de
Ruiz, Cristina
Espinosa Blay, Lluís
Bigas Salvans, Anna
Ramón y Cajal Agüeras, Santiago
Fernández Ruiz, Pedro Luis
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
Thomson, Timothy M.
Paciucci Barzanti, Rosanna
Keywords: Càncer de pròstata
Marcadors tumorals
Prostate cancer
Tumor markers
Issue Date: 31-Mar-2014
Publisher: BioMed Central
Abstract: Background PTOV1 is an adaptor protein with functions in diverse processes, including gene transcription and protein translation, whose overexpression is associated with a higher proliferation index and tumor grade in prostate cancer (PC) and other neoplasms. Here we report its interaction with the Notch pathway and its involvement in PC progression. Methods Stable PTOV1 knockdown or overexpression were performed by lentiviral transduction. Protein interactions were analyzed by co-immunoprecipitation, pull-down and/or immunofluorescence. Endogenous gene expression was analyzed by real time RT-PCR and/or Western blotting. Exogenous promoter activities were studied by luciferase assays. Gene promoter interactions were analyzed by chromatin immunoprecipitation assays (ChIP). In vivo studies were performed in the Drosophila melanogaster wing, the SCID-Beige mouse model, and human prostate cancer tissues and metastasis. The Excel package was used for statistical analysis [...].
Note: Reproducció del document publicat a:
It is part of: Molecular Cancer, 2014, vol. 13, num. 74, p. 1-16
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ISSN: 1476-4598
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Fonaments Clínics)

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