Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/96505
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gallego, Ana | - |
dc.contributor.author | Jáuregui Pallarés, Olga | - |
dc.contributor.author | Moyano Claramunt, Elisabet | - |
dc.contributor.author | Palazón Barandela, Javier | - |
dc.contributor.author | Casals i Ribes, Isidre | - |
dc.contributor.author | Bonfill Baldrich, Ma. Mercedes | - |
dc.date.accessioned | 2016-03-15T15:20:32Z | - |
dc.date.available | 2016-03-15T15:20:32Z | - |
dc.date.issued | 2015-02-04 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | http://hdl.handle.net/2445/96505 | - |
dc.description.abstract | Since the recent discovery of taxol and other taxanes in Corylus avellana, this plant species has attracted interest as a potential new source of these compounds. However, its low taxane content in comparison with Taxus spp. has restricted research to analytical identification or global quantitation. A feasible and sensitive method based on liquid chromatography-tandem mass spectrometry using a triple quadrupole analyzer was developed for the analysis of taxol and four other taxanes in a Corylus avellana cell suspension medium. Taxanes were extracted from the cell culture medium with dichloromethane and analyzed using electrospray ionization and quantified by multiple-reaction monitoring mode. Methanol and matrix-matching calibration curves using docetaxel as the internal standard were analyzed. Linearity was confirmed over the whole calibration range (0.3-2.1 μg mL−1). The inter- and intra-day precision of taxanes ranged from 80% to 120% and the recovery rates were higher than 80%. Limits of detection were between 0.24-38 ng mL−1 and the limits of quantification were between 0.8-125 ng mL−1. The low detection and quantitation values obtained allowed us to detect small quantities of the released taxanes (120 ng mL−1 of B, 151 ng mL−1 of CF and 105 ng mL−1 of T), which correspond to about 0.5 ng mL−1 of each taxane, in the 20 mL Corylus avellana cell suspension culture medium extracted, even at the beginning of the culture. These results were confirmed by high resolution mass spectrometry. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Royal Society of Chemistry | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1039/C5RA00803D | - |
dc.relation.ispartof | RSC Advances, 2015, vol. 5 , num. 23, p. 17976-17983 | - |
dc.relation.uri | http://dx.doi.org/10.1039/C5RA00803D | - |
dc.rights | cc-by (c) Gallego, A. et al., 2015 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) | - |
dc.subject.classification | Cromatografia de líquids | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.other | Liquid chromatography | - |
dc.subject.other | Mass spectrometry | - |
dc.title | Optimization of a liquid chromatography-tandem mass spectrometry method for the quantification of traces of taxanes in a Corylus avellana cell suspension medium | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 646733 | - |
dc.date.updated | 2016-03-15T15:20:37Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
646733.pdf | 589.74 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License