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Title: The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
Author: García-Basteiro, Alberto L.
Ismail, Mamudo R.
Carrilho, Carla
Ussene, Esperança
Castillo, Paola
Chitsungo, Dércio
Rodríguez, Cristina
Lovane, Lucilia
Vergara Gómez, Andrea
López Varela, Elisa
Mandomando, Inácio
Lorenzoni, Cesaltina
Ordi i Majà, Jaume
Menéndez, Clara
Bassat Orellana, Quique
Martínez Yoldi, Miguel Julián
Keywords: Bacteriologia
Microbiologia mèdica
Malalties infeccioses
Medical microbiology
Communicable diseases
Issue Date: 10-Feb-2016
Publisher: Nature Publishing Group
Abstract: The extent to which the Xpert MTB/RIF (Gene Xpert) contributes to tuberculosis (TB) diagnosis in samples other than sputum and cerebrospinal fluid remains uncertain. We aimed to assess the role of Xpert MTB/RIF for detecting M. tuberculosis in post-mortem tissues. We conducted a study among 30 complete diagnostic autopsies (CDA) performed at the Maputo Central Hospital (Mozambique). Lung tissues were screened for TB in all cases. In addition other tissues were tested when compatible lesions were identified in the histological exam. We used in-house real time PCR and LAMP assays to confirm the presence of M. tuberculosis DNA. The diagnosis of tuberculosis at death was established based on microbiological and histopathological results. Eight out of 30 cases (26.7%) were diagnosed of tuberculosis. Xpert had a sensitivity to detect TB in lung tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7% (95% CI: 78.1-99.9). In-house DNA amplification methods and Xpert showed 93.6% concordance for lung tissue and 100% concordance for brain and liver tissues. The final cause of death was attributable to tuberculosis in four cases. Xpert MTB/RIF may represent a valuable, easy-to perform technique for post-mortem TB diagnosis.
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It is part of: Scientific Reports, 2016, vol. 6, num. 20703
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (ISGlobal)
Articles publicats en revistes (Fonaments Clínics)

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