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Title: Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin
Author: Nithichanon, Arnone
Rinchai, Darawan
Gori, Alessandro
Lassaux, Patricia
Peri, Claudio
Conchillio-Solé, Oscar
Ferrer-Navarro, Mario
Gourlay, Louise J.
Nardini, Marco
Vila Estapé, Jordi
Daura, Xavier
Colombo, Giorgio
Bolognesi, Martino
Lertmemonkolchai, Ganjana
Keywords: Cèl·lules T
Síntesi de pèptids
Cèl·lules B
Anticossos monoclonals
T cells
Peptide synthesis
B cells
Monoclonal antibodies
Issue Date: 2015
Publisher: Public Library of Science (PLoS)
Abstract: Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.
Note: Reproducció del document publicat a:
It is part of: PLoS Neglected Tropical Diseases, 2015, vol. 9, num. 7
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ISSN: 1935-2735
Appears in Collections:Articles publicats en revistes (ISGlobal)
Articles publicats en revistes (Fonaments Clínics)

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