Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/96977
Title: Prevalence and molecular characterization of methicillin-resistant S. aureus ST398 resistant to tetracycline at a Spanish hospital over 12 years
Author: Camoez, Mariana
Sierra, Josep M.
Pujol Rojo, Miquel
Hornero, Ana
Martin, Rogelio
Domínguez Luzón, Ma. Ángeles (María Ángeles)
Keywords: Clonatge
Antibiòtics
Microxips d'ADN
Staphylococcus aureus
Cloning
Antibiotics
DNA microarrays
Staphylococcus aureus
Issue Date: 2013
Publisher: Public Library of Science (PLoS)
Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) ST398, associated with livestock animals, was described in 2003 as a new lineage infecting or colonizing humans. We evaluated the prevalence and molecular characteristics of MRSA ST398 isolated in the Hospital Universitari de Bellvitge from January 2000 to June 2011. Tetracycline resistant (Tet-R) MRSA isolates from single patients (pts) were screened by SmaI-pulsed field gel electrophoresis (PFGE). Nontypable MRSA strains by SmaI (NTSmaI)-MRSA were further analysed by ApaI-PFGE, spa, SCCmec, agr, MLST typing, and by DNA microarray hybridization. Among 164 pts harboring Tet-R MRSA, NTSmaI-MRSA ST398-agrI was found in 33 pts (20%). Although the first pt was detected in 2003, 22/33 pts (67%) were registered in the 2010-2011 period. Ten pts (30%) were infected and cancer was the most frequent underlying disease. In one case, death was due to MRSA-ST398-related infection. Five pulsotypes (A-E) were detected using ApaI-PFGE, with type A accounting for 76% of the strains. The majority of the studied isolates presented spa type t011 (70%) and SCCmec type V (88%). One strain was spa negative both by PCR and microarray analysis. Forty-nine percent of the studied isolates showed resistance to 3 or more antibiotic classes, in addition to beta-lactams. Ciprofloxacin resistance was 67%. Tet-R was mediated by tet(M) and tet(K) in 26 isolates. All isolates lacked Panton-Valentine Leukocidin production, as well as other significant toxins. This study displays the molecular features of MRSA-ST398 clone and shows the increase in tetracycline resistance together with arise in MRSA-ST398 isolates infecting or colonizing patients in our clinical setting.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0072828
It is part of: PLoS One, 2013, vol. 8, num. 9
Related resource: http://dx.doi.org/10.1371/journal.pone.0072828
URI: http://hdl.handle.net/2445/96977
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Patologia i Terapèutica Experimental)

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