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http://hdl.handle.net/2445/97403
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DC Field | Value | Language |
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dc.contributor.author | Le Roux, Anabel-Lise | - |
dc.contributor.author | Castro, Bruno | - |
dc.contributor.author | Garbacik, Erik T. | - |
dc.contributor.author | García-Parajó, Maria F. | - |
dc.contributor.author | Pons Vallès, Miquel | - |
dc.date.accessioned | 2016-04-14T10:23:17Z | - |
dc.date.issued | 2016-03-08 | - |
dc.identifier.issn | 2365-6549 | - |
dc.identifier.uri | http://hdl.handle.net/2445/97403 | - |
dc.description.abstract | The proto-oncogene tyrosine-protein kinase Src is a key ele- ment of signaling cascades involved in the invasive and meta- stasis-forming capacity of cancer cells. While membrane ty- rosine-kinase receptors are known to dimerize, Src is classified as a non-receptor kinase and assumed to remain always mono- meric. Here we demonstrate the formation of stable dimers by the first domains of myristoylated Src previously shown to be sufficient for Src trafficking. Src dimers fused to green fluo- rescent protein (GFP) on supported lipid bilayers were identi- fied using single-molecule photobleaching experiments. Com- petition with a protein containing only native Src domains without GFP confirms that dimerization is a previously over- looked intrinsic property of Src. Dimerization is concomitant to membrane binding by the myristoylated forms of Src and may constitute a new regulation layer for the Src oncogene. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-VCH | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1002/slct.201600117 | - |
dc.relation.ispartof | ChemistrySelect, 2016, vol. 1, num. 4, p. 642-647 | - |
dc.relation.uri | http://dx.doi.org/10.1002/slct.201600117 | - |
dc.rights | cc by-nc (c) Le Roux et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nd/3.0/es/ | * |
dc.source | Articles publicats en revistes (Química Inorgànica i Orgànica) | - |
dc.subject.classification | Bicapes lipídiques | - |
dc.subject.classification | Proteïnes quinases | - |
dc.subject.classification | Transducció de senyal cel·lular | - |
dc.subject.classification | Membranes cel·lulars | - |
dc.subject.other | Lipid bilayers | - |
dc.subject.other | Protein kinases | - |
dc.subject.other | Cellular signal transduction | - |
dc.subject.other | Cell membranes | - |
dc.title | Single molecule fluorescence reveals dimerization of myristoylated Src N-terminal region on supported lipid bilayers | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 660240 | - |
dc.date.updated | 2016-04-14T10:23:22Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) |
Files in This Item:
File | Description | Size | Format | |
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660240.pdf | 1.49 MB | Adobe PDF | View/Open |
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