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http://hdl.handle.net/2445/97581
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DC Field | Value | Language |
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dc.contributor.author | Dropmann, Anne | - |
dc.contributor.author | Dediulia, Tatjana | - |
dc.contributor.author | Breitkopf-Heinlein, Katja | - |
dc.contributor.author | Korhonen, Hanna | - |
dc.contributor.author | Janicot, Michel | - |
dc.contributor.author | Weber, Susanne N. | - |
dc.contributor.author | Thomas, Maria | - |
dc.contributor.author | Piiper, Albrecht | - |
dc.contributor.author | Bertran Rodríguez, Esther | - |
dc.contributor.author | Fabregat Romero, Isabel | - |
dc.contributor.author | Abshagen, Kerstin | - |
dc.contributor.author | Hess, Jochen | - |
dc.contributor.author | Angel, Peter | - |
dc.contributor.author | Coulouarn, Cédric | - |
dc.contributor.author | Dooley, Steven | - |
dc.contributor.author | Meindl-Beinker, Nadja M. | - |
dc.date.accessioned | 2016-04-18T15:07:42Z | - |
dc.date.available | 2016-04-18T15:07:42Z | - |
dc.date.issued | 2016-01-21 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | http://hdl.handle.net/2445/97581 | - |
dc.description.abstract | TGF-β1 is a major player in chronic liver diseases promoting fibrogenesis and tumorigenesis through various mechanisms. The expression and function of TGF-β2 have not been investigated thoroughly in liver disease to date. In this paper, we provide evidence that TGF-β2 expression correlates with fibrogenesis and liver cancer development. Using quantitative realtime PCR and ELISA, we show that TGF-β2 mRNA expression and secretion increased in murine HSCs and hepatocytes over time in culture and were found in the human-derived HSC cell line LX-2. TGF-β2 stimulation of the LX-2 cells led to upregulation of the TGF-β receptors 1, 2, and 3, whereas TGF-β1 treatment did not alter or decrease their expression. In liver regeneration and fibrosis upon CCl4 challenge, the transient increase of TGF-β2 expression was accompanied by TGF-β1 and collagen expression. In bile duct ligation-induced fibrosis, TGF-β2 upregulation correlated with fibrotic markers and was more prominent than TGF-β1 expression. Accordingly, MDR2-KO mice showed significant TGF-β2 upregulation within 3 to 15 months but minor TGF-β1 expression changes. In 5 of 8 hepatocellular carcinoma (HCC)/hepatoblastoma cell lines, relatively high TGF-β2 expression and secretion were observed, with some cell lines even secreting more TGF-β2 than TGF-β1. TGF-β2 was also upregulated in tumors of TGFα/cMyc and DEN-treated mice. The analysis of publically available microarray data of 13 human HCC collectives revealed considerable upregulation of TGF-β2 as compared to normal liver. Our study demonstrates upregulation of TGF-β2 in liver disease and suggests TGF-β2 as a promising therapeutic target for tackling fibrosis and HCC. | - |
dc.format.extent | 20 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Impact Journals | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.18632/oncotarget.6967 | - |
dc.relation.ispartof | Oncotarget, 2016, vol. 7, num. 15, p. 19499-19518 | - |
dc.relation.uri | http://dx.doi.org/10.18632/oncotarget.6967 | - |
dc.rights | cc-by (c) Dropmann, Anne et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Malalties del fetge | - |
dc.subject.classification | Fibrosi quística | - |
dc.subject.classification | Ratolins (Animals de laboratori) | - |
dc.subject.classification | Regeneració (Biologia) | - |
dc.subject.other | Liver diseases | - |
dc.subject.other | Cystic fibrosis | - |
dc.subject.other | Mice (Laboratory animals) | - |
dc.subject.other | Regeneration (Biology) | - |
dc.title | TGF-β1 and TGF-β2 abundance in liver diseases of mice and men | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 657826 | - |
dc.date.updated | 2016-04-18T15:07:48Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26799667 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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657826.pdf | 2.65 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License