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http://hdl.handle.net/2445/98051
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DC Field | Value | Language |
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dc.contributor.author | Para, B. V. (Bianca Veronica) | - |
dc.contributor.author | Núñez Burcio, Oscar | - |
dc.contributor.author | Moyano Morcillo, Encarnación | - |
dc.contributor.author | Galcerán Huguet, M. Teresa | - |
dc.date.accessioned | 2016-04-29T11:18:30Z | - |
dc.date.available | 2016-04-29T11:18:30Z | - |
dc.date.issued | 2006-05-30 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | http://hdl.handle.net/2445/98051 | - |
dc.description.abstract | Conditions for the separation and determination of benzalkonium chloride (BAC) homologues by CE with UV-detection and CE coupled to MS (IT) using electrospray as ionization source were established. The separation was performed using fused-silica capillaries of 50 μm id and 100 mM acetic acid-ammonium acetate buffer solution at pH 4.5 with 80% of ACN as carrier electrolyte. CE-MS coupling parameters were optimized and methanol-10 mM acetic acid (90:10 v/v) was selected as sheath liquid. Detection limits, based on an S/N of 3:1, were calculated, and values between 0.8 and 1.3 mg/L with CE-ESI/MS and around 0.5 mg/L with CE-ESI-MS/MS, using hydrodynamic injection (15 s, 3.5 kPa), were obtained. Good run-to-run and day-to-day precisions on concentration were achieved with RSDs lower than 8%. Quantitative analysis was carried out by the internal standard method and the calibration curves showed good linearities (r2>0.98). The CE-ESI-MS/MS method was successfully applied to the analysis of BAC in different ophthalmic solutions, allowing the direct determination, identification and confirmation of the BAC homologues presented in these samples. | - |
dc.format.extent | 26 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-VCH | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1002/elps.200500716 | - |
dc.relation.ispartof | Electrophoresis, 2006, vol. 27, num. 11, p. 2225-2232 | - |
dc.relation.uri | http://dx.doi.org/10.1002/elps.200500716 | - |
dc.rights | (c) Wiley-VCH, 2006 | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Electroforesi capil·lar | - |
dc.subject.other | Capillary electrophoresis | - |
dc.title | Análisis of Benzalkonium chloride by capillary electrophoresis-electrospray-Tandem mass spectrometry | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 536299 | - |
dc.date.updated | 2016-04-29T11:18:36Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
File | Description | Size | Format | |
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536299.pdf | 4.44 MB | Adobe PDF | View/Open |
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