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http://hdl.handle.net/2445/98697
Title: | Ultra rapid in vivo screening for anti-Alzheimer anti-amyloid drugs |
Author: | Espargaró Colomé, Alba Medina, Aina Di Pietro, O. Muñoz-Torrero López-Ibarra, Diego Sabaté Lagunas, Raimon |
Keywords: | Malaltia d'Alzheimer Pèptids Agregació (Química) Disseny de medicaments Alzheimer's disease Peptides Aggregation (Chemistry) Drug design |
Issue Date: | 22-Mar-2016 |
Publisher: | Nature Publishing Group |
Abstract: | More than 46 million people worldwide suffer from Alzheimer's disease. A large number of potential treatments have been proposed; among these, the inhibition of the aggregation of amyloid β-peptide (Aβ), considered one of the main culprits in Alzheimer's disease. Limitations in monitoring the aggregation of Aβ in cells and tissues restrict the screening of anti-amyloid drugs to in vitro studies in most cases. We have developed a simple but powerful method to track Aβ aggregation in vivo in realtime, using bacteria as in vivo amyloid reservoir. We use the specific amyloid dye Thioflavin-S (Th-S) to stain bacterial inclusion bodies (IBs), in this case mainly formed of Aβ in amyloid conformation. Th-S binding to amyloids leads to an increment of fluorescence that can be monitored. The quantification of the Th-S fluorescence along the time allows tracking Aβ aggregation and the effect of potential antiaggregating agents. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1038/srep23349 |
It is part of: | Scientific Reports, 2016, vol. 6, p. 23349 |
URI: | http://hdl.handle.net/2445/98697 |
Related resource: | http://dx.doi.org/10.1038/srep23349 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica) Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB)) |
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