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http://hdl.handle.net/2445/99326
Title: | Proline cis-trans isomerization and its implications for the dimerization of analogues of cyclopeptide stylostatin 1: a combined computational and experimental study |
Author: | López Martínez, Carlos Flores-Morales, P. Cruz, M. Gonzalez, Teresa Feliz Rodenas, Miguel Diez Pascual, Anna Campanera Alsina, Josep Maria |
Keywords: | Química orgànica Pèptids Organic chemistry Peptides |
Issue Date: | 18-Mar-2016 |
Publisher: | Royal Society of Chemistry |
Abstract: | Cis and trans proline conformers are often associated with dramatic changes in the biological function of peptides. A slow equilibrium between cis and trans Ile-Pro amide bond conformers occurs in constrained derivatives of the native marine cyclic heptapeptide stylostatin 1 (cyclo-(NSLAIPF)), a potential anticancer agent. In this work, four cyclopeptides, cyclo-(NSTAIPF), cyclo-(KSTAIPF), cyclo-(RSTAIPF) and cyclo-(DSTAIPF), which are structurally related to stylostatin 1, are experimentally and computationally examined in order to assess the effect of residue mutations on the cis-trans conformational ratio and the apparent capacity to form dimeric aggregates. Primarily, cyclo-(KSTAIPF) and cyclo-(RSTAIPF) showed specific trends in circular dichroism, MALDI-TOF and HPLC purification experiments, which suggests the occurrence of peptide dimerization. Meanwhile, the NMR spectrum of cyclo-(KSTAIPF) indicates that this cyclopeptide exists in the two slow-exchange families of conformations mentioned above. Molecular dynamics simulations combined with quantum mechanical calculations have shed light on the factors governing the cis/trans conformational ratio. In particular, we have found that residue mutations affect the internal hydrogen bond pattern which ultimately tunes the cis/trans conformational ratio and that only trans conformers are capable of aggregating due to the shape complementarity of the two subunits. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1039/c5cp05937b |
It is part of: | Physical Chemistry Chemical Physics, 2016, vol. 18, num. 18, p. 12755-12767 |
URI: | http://hdl.handle.net/2445/99326 |
Related resource: | http://dx.doi.org/10.1039/c5cp05937b |
ISSN: | 1463-9076 |
Appears in Collections: | Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB)) Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) |
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