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Title: | Epigenome-Wide Meta-Analysis of Methylation in Children Related to Prenatal NO2 Air Pollution Exposure |
Author: | Gruzieva, Olena Xu, Cheng-Jian Breton, Carrie V. Annesi-Maesano, Isabella Antó i Boqué, Josep Maria Auffray, Charles Ballereau, Stephane Bellander, Tom Bousquet, Jean Bustamante Pineda, Mariona Charles, Marie-Aline Kluizenaar, Yvonne de Dekker, Herman T. den Duijts, Liesbeth Felix, Janine F. Gehring, Ulrike Guxens, Mònica Jaddoe, Vincent W. Jankipersadsing, Soesma A. Merid, Simon Kebede Kere, Juha Kumar, Ashish Lemonnier, Nathanael Lepeule, Johanna Nystad, Wenche Page, Christian Magnus Panasevich, Sviatlana Postma, Dirkje S. Slama, Rémy Sunyer Deu, Jordi Soderhall, Cilla Yao, Jin London, Stephanie J. Pershagen, Göran Koppelman, Gerard H. Melén, Erik |
Keywords: | Contaminació Medicina prenatal Pollution Prenatal medicine |
Issue Date: | 22-Jul-2016 |
Publisher: | National Institute of Environmental Health Sciences |
Abstract: | BACKGROUND: Prenatal exposure to air pollution is considered to be associated with adverse effects on child health. This may partly be mediated by mechanisms related to DNA methylation. OBJECTIVES: We investigated associations between exposure to air pollution, using nitrogen dioxide (NO2) as marker, and epigenome-wide cord blood DNA methylation. METHODS: We meta-analyzed the associations between NO2 exposure at residential addresses during pregnancy and cord blood DNA methylation (Illumina 450K) in four European and North-American studies (n=1,508) with subsequent look-up analyses in children aged 4 (n=733) and 8 (n=786) years. Additionally, we applied a literature-based candidate approach for antioxidant and anti-inflammatory genes. To assess influence of exposure at the transcriptomics level, we related mRNA expression in blood cells to NO2 exposure in 4- (n=111) and 16-year-olds (n=239). RESULTS: We found epigenome-wide significant associations (false discovery rate (FDR) p<0.05) between maternal NO2 exposure during pregnancy and DNA methylation in newborns for 3 CpG sites in mitochondria-related genes: cg12283362 (LONP1), cg24172570 (3.8 kbp upstream of HIBADH), and cg08973675 (SLC25A28). The associations with cg08973675 methylation were also significant in the older children. Further analysis of antioxidant and anti-inflammatory genes revealed differentially methylated CpGs in CAT and TPO in newborns (FDR p<0.05). NO2 exposure at the time of biosampling in childhood had significant impact on CAT and TPO expression. CONCLUSIONS: NO2 exposure during pregnancy was associated with differential offspring DNA methylation in mitochondria-related genes. Exposure to NO2 was also linked to differential methylation as well as expression of genes involved in antioxidant defense pathways. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1289/EHP36 |
It is part of: | Environmental Health Perspectives, 2016 |
URI: | http://hdl.handle.net/2445/101564 |
Related resource: | http://dx.doi.org/10.1289/EHP36 |
ISSN: | 0091-6765 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Publicacions de projectes de recerca finançats per la UE |
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