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http://hdl.handle.net/2445/101576
Title: | Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids |
Author: | Pazos Pérez, Nicolas Pazos, Elena Catala, Carme Mir Simon, Bernat Gómez de Pedro, Sara Sagales, Juan Villanueva, Carlos Vila Estapé, Jordi Soriano Viladomiu, Alex García de Abajo, F. Javier Álvarez Puebla, Ramón A. |
Keywords: | Malalties bacterianes Nanopartícules Bacterial diseases Nanoparticles |
Issue Date: | 1-Jul-2016 |
Publisher: | Springer Nature |
Abstract: | Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large samples of blood (~1 milliliter) extending over 24-72 hours. This delay seriously compromises the health of patients. Here we demonstrate a fast microorganism optical detection system for the exhaustive identification and quantification of pathogens in volumes of biofluids with clinical relevance (~1 milliliter) in minutes. We drive each type of bacteria to accumulate antibody functionalized SERS-labelled silver nanoparticles. Particle aggregation on the bacteria membranes renders dense arrays of inter-particle gaps in which the Raman signal is exponentially amplified by several orders of magnitude relative to the dispersed particles. This enables a multiplex identification of the microorganisms through the molecule-specific spectral fingerprints. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1038/srep29014 |
It is part of: | Scientific Reports, 2016, vol. 6, num. , p. 29014 |
URI: | http://hdl.handle.net/2445/101576 |
Related resource: | http://dx.doi.org/10.1038/srep29014 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Medicina) Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Fonaments Clínics) Publicacions de projectes de recerca finançats per la UE |
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