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http://hdl.handle.net/2445/101598
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DC Field | Value | Language |
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dc.contributor.author | Dentesano, Guido | - |
dc.contributor.author | Straccia, Marco | - |
dc.contributor.author | Ejarque Ortiz, Aroa | - |
dc.contributor.author | Tusell Puigbert, José Ma. | - |
dc.contributor.author | Serratosa i Serdà, Joan | - |
dc.contributor.author | Saura Martí, Josep | - |
dc.contributor.author | Solà i Subirana, Carme | - |
dc.date.accessioned | 2016-09-06T16:23:37Z | - |
dc.date.available | 2016-09-06T16:23:37Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1742-2094 | - |
dc.identifier.uri | http://hdl.handle.net/2445/101598 | - |
dc.description.abstract | Background: In physiological conditions, it is postulated that neurons control microglial reactivity through a series of inhibitory mechanisms, involving either cell contact-dependent, soluble-factor-dependent or neurotransmitter-associated pathways. In the current study, we focus on CD200R1, a microglial receptor involved in one of these cell contact-dependent mechanisms. CD200R1 activation by its ligand, CD200 (mainly expressed by neurons in the central nervous system),is postulated to inhibit the pro-inflammatory phenotype of microglial cells, while alterations in CD200-CD200R1 signalling potentiate this phenotype. Little is known about the regulation of CD200R1 expression in microglia or possible alterations in the presence of pro-inflammatory stimuli. Methods: Murine primary microglial cultures, mixed glial cultures from wild-type and CCAAT/enhancer binding protein β (C/EBPβ)-deficient mice, and the BV2 murine cell line overexpressing C/EBPβ were used to study the involvement of C/EBPβ transcription factor in the regulation of CD200R1 expression in response to a proinflammatory stimulus (lipopolysaccharide (LPS)). Binding of C/EBPβ to the CD200R1 promoter was determined by quantitative chromatin immunoprecipitation (qChIP). The involvement of histone deacetylase 1 in the control of CD200R1 expression by C/EBPβ was also determined by co-immunoprecipitation and qChIP. Results: LPS treatment induced a decrease in CD200R1 mRNA and protein expression in microglial cells, an effect that was not observed in the absence of C/EBPβ. C/EBPβ overexpression in BV2 cells resulted in a decrease in basal CD200R1 mRNA and protein expression. In addition, C/EBPβ binding to the CD200R1 promoter was observed in LPS-treated but not in control glial cells, and also in control BV2 cells overexpressing C/EBPβ. Finally, we observed that histone deacetylase 1 co-immunoprecipitated with C/EBPβ and showed binding to a C/EBPβ consensus sequence of the CD200R1 promoter in LPS-treated glial cells. Moreover, histone deacetylase 1 inhibitors reversed the decrease in CD200R1 expression induced by LPS treatment. Conclusions: CD200R1 expression decreases in microglial cells in the presence of a pro-inflammatory stimulus, an effect that is regulated, at least in part, by C/EBPβ. Histone deacetylase 1 may mediate C/EBPβ inhibition of CD200R1 expression, through a direct effect on C/EBPβ transcriptional activity and/or on chromatin structure. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1186/1742-2094-9-165 | - |
dc.relation.ispartof | Journal of Neuroinflammation, 2012, vol. 9, num. 165 | - |
dc.relation.uri | http://dx.doi.org/10.1186/1742-2094-9-165 | - |
dc.rights | cc-by (c) Dentesano, Guido et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Neurociències | - |
dc.subject.classification | Neurobiologia | - |
dc.subject.classification | Neuròglia | - |
dc.subject.classification | Teixit nerviós | - |
dc.subject.classification | Cultiu de teixits | - |
dc.subject.other | Neurosciences | - |
dc.subject.other | Neurobiology | - |
dc.subject.other | Neuroglia | - |
dc.subject.other | Nerve tissue | - |
dc.subject.other | Tissue culture | - |
dc.title | Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 617319 | - |
dc.date.updated | 2016-09-06T16:23:42Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22776069 | - |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
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