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http://hdl.handle.net/2445/102231
Title: | Artificially-induced organelles are optimal targets for optical trapping experiments in living cells |
Author: | López-Quesada, Carol Fontaine, Anne-Sophie Farré Flaquer, Arnau Joseph, Minu Selva Sánchez, Javier Egea Guri, Gustavo Ludevid, Dolors Martín Badosa, Estela Montes Usategui, Mario |
Keywords: | Processament òptic de dades Cèl·lules Interacció cel·lular Optical data processing Cells Cell interaction |
Issue Date: | 30-May-2014 |
Publisher: | Optical Society of America |
Abstract: | Optical trapping supplies information on the structural, kinetic or rheological properties of inner constituents of the cell. However, the application of significant forces to intracellular objects is notoriously difficult due to a combination of factors, such as the small difference between the refractive indices of the target structures and the cytoplasm. Here we discuss the possibility of artificially inducing the formation of spherical organelles in the endoplasmic reticulum, which would contain densely packed engineered proteins, to be used as optimized targets for optical trapping experiments. The high index of refraction and large size of our organelles provide a firm grip for optical trapping and thereby allow us to exert large forces easily within safe irradiation limits. This has clear advantages over alternative probes, such as subcellular organelles or internalized synthetic beads. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1364/BOE.5.001993 |
It is part of: | Biomedical Optics Express, 2014, vol. 5, num. 7, p. 1993-2008 |
URI: | http://hdl.handle.net/2445/102231 |
Related resource: | http://dx.doi.org/10.1364/BOE.5.001993 |
ISSN: | 2156-7085 |
Appears in Collections: | Articles publicats en revistes (Física Aplicada) |
Files in This Item:
File | Description | Size | Format | |
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649091.pdf | 2.79 MB | Adobe PDF | View/Open |
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