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Title: | Reduced adenosine uptake and its contribution to signaling that mediates profibrotic activation in renal tubular epithelial cells: implication in diabetic nephropathy. |
Author: | Kretschmar, Catalina Oyarzún, Carlos Villablanca, Cristopher Jaramillo, Catherinne Alarcón, Sebastián Perez, Gustavo Díaz-Encarnación, Montserrat M. M. Pastor Anglada, Marçal Garrido, Wallys Quezada, Claudia San Martín, Rody |
Keywords: | Diabetis Cèl·lules epitelials Adenosina Diabetes Epithelial cells Adenosine |
Issue Date: | 25-Jan-2016 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Altered nucleoside levels may be linked to pathogenic signaling through adenosine recep- tors. We hypothesized that adenosine dysregulation contributes to fibrosis in diabetic kid- ney disease. Our findings indicate that high glucose levels and experimental diabetes decreased uptake activity through the equilibrative nucleoside transporter 1 (ENT1) in proxi- mal tubule cells. In addition, a correlation between increased plasma content of adenosine and a marker of renal fibrosis in diabetic rats was evidenced. At the cellular level, exposure of HK2 cells to high glucose, TGF- β and the general adenosine receptor agonist NECA, induced the expression of profibrotic cell activation markers α -SMA and fibronectin. These effects can be avoided by using a selective antagonist of the adenosine A 3 receptor subtype in vitro. Furthermore, induction of fibrosis marker α -SMA was prevented by the A 3 receptor antagonist in diabetic rat kidneys. In conclusion, we evidenced the contribution of purinergic signaling to renal fibrosis in experimental diabetic nephropathy. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0147430 |
It is part of: | PLoS One, 2016, vol. 11, num. 1, p. e0147430 |
URI: | http://hdl.handle.net/2445/104525 |
Related resource: | https://doi.org/10.1371/journal.pone.0147430 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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