Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorShitara, Danielle-
dc.contributor.authorTell Martí, Gemma-
dc.contributor.authorBadenas Orquin, Celia-
dc.contributor.authorEnokihara, MM.-
dc.contributor.authorAlós i Hernández, Llúcia-
dc.contributor.authorLarque, A.B.-
dc.contributor.authorMichalany, Nilceo-
dc.contributor.authorPuig Butillé, Joan Anton-
dc.contributor.authorCarrera Álvarez, Cristina-
dc.contributor.authorMalvehy, J. (Josep)-
dc.contributor.authorPuig i Sardà, Susana-
dc.contributor.authorBagatin, E.-
dc.description.abstractIntroduction Melanoma origin has always been a debated subject, as well as the role of adjacent melanocytic nevi. Epidemiological and histopathological studies point to melanomas arising either de novo or from a nevus. Methods Sixty-one melanomas found in association with a preexisting nevus were microdissected, after careful selection of cell subpopulations and submitted to Sanger sequencing of the BRAF, NRAS, C-KIT, PPP6C, STK19 and RAC1 genes. Each gene was evaluated twice in all samples by sequencing or by sequencing and another confirmation method, allele-specific fluorescent polymerase chain reaction (PCR) and capillary electrophoresis detection, or by SNaPshot Analysis. Only mutations confirmed via two different molecular methods or twice by sequencing were considered positive. Results The majority of cases presented concordance of mutational status between melanoma and the associated nevus for all 6 genes (40/60; 66.7%). Nine cases presented concomitant BRAF and NRAS mutations, including one case, in which both the melanoma and the adjacent nevus harbored V600E and Q61K double mutations. In two cases, both melanoma and associated nevus, located on acral sites were BRAF mutated, including an acral lentiginous melanoma. Conclusions This is the largest nevus-associated melanoma series molecularly evaluated to our knowledge. The majority of melanomas and adjacent nevi in our sample share the same mutational profile, corroborating the theory that the adjacent nevus and melanoma are clonally related and that melanoma originated within a nevus.-
dc.format.extent20 p.-
dc.relation.isformatofVersió postprint del document publicat a:
dc.relation.ispartofBritish Journal of Dermatology, 2015, vol. 173, num. 3, p. 671-680-
dc.rights(c) British Association of Dermatologists, 2015-
dc.subject.classificationMutació (Biologia)-
dc.subject.otherMutation (Biology)-
dc.titleMutational status of nevus-associated melanomas.-
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)

Files in This Item:
File Description SizeFormat 
667486.pdf976.66 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.