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http://hdl.handle.net/2445/107055
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DC Field | Value | Language |
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dc.contributor.author | Aviñó Andrés, Anna | - |
dc.contributor.author | Mazzini, Stefania | - |
dc.contributor.author | Fàbrega i Claveria, Ma. Carme | - |
dc.contributor.author | Peñalver, Pablo | - |
dc.contributor.author | Gargallo Gómez, Raimundo | - |
dc.contributor.author | Morales, J.C. | - |
dc.contributor.author | Eritja i Casadellà, Ramon | - |
dc.date.accessioned | 2017-02-16T11:54:21Z | - |
dc.date.available | 2017-10-02T22:01:18Z | - |
dc.date.issued | 2016-10-02 | - |
dc.identifier.issn | 0304-4165 | - |
dc.identifier.uri | http://hdl.handle.net/2445/107055 | - |
dc.description.abstract | Background Guanine-rich oligonucleotides are capable of forming tetrahelical structures known as G-quadruplexes with interesting biological properties. We have investigated the effects of site-specific substitution in the loops and in the tetrads model G-quadruplexes using thymine glycol nucleic acid (GNA) units, l-thymidine and 8-Br-2′-deoxyguanosine. Methods Modified oligonucleotides were chemically synthesized and spectroscopic techniques were used to determine the relative stability of the modified G-quadruplex. The double 8-BrdG-modified quadruplexes were further characterized by Nuclear Magnetic Resonance. Binding to thrombin of selected quadruplex was analyzed by gel electrophoresis retention assay. Results The most interesting results were found with a 8-bromoG substitution that had the larger stabilization of the quadruplex. NMR studies indicate a tight relationship between the loops and the tetrads to accommodate 8-bromoG modifications within the TBA. Conclusions The substitutions of loop positions with GNA T affect the TBA stability except for single modification in T7 position. Single l-thymidine substitutions produced destabilization of TBA. Larger changes on quadruplex stability are observed with the use of 8-bromoG finding a single substitution with the highest thermal stabilization found in thrombin binding aptamers modified at the guanine residues and having good affinity for thrombin. Double 8-BrdG modification in anti positions of different tetrads produce a conformational flip from syn to anti conformation of 8-Br-dG to favor loop-tetrad interaction and preserve the overall TBA stability. | - |
dc.format.extent | 29 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.bbagen.2016.09.030 | - |
dc.relation.ispartof | Biochimica et Biophysica Acta-General Subjects, 2017, vol. 1861, num. 5, Part B, p. 1205-1212 | - |
dc.relation.uri | https://doi.org/10.1016/j.bbagen.2016.09.030 | - |
dc.rights | cc-by-nc-nd (c) Elsevier B.V., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Biologia molecular | - |
dc.subject.classification | Bioquímica | - |
dc.subject.classification | Oligonucleòtids | - |
dc.subject.classification | G-estructures | - |
dc.subject.other | Molecular biology | - |
dc.subject.other | Biochemistry | - |
dc.subject.other | Oligonucleotides | - |
dc.subject.other | G-structures | - |
dc.title | The effect of l-thymidine, acyclic thymine and 8-bromoguanine on the stability of model G-quadruplex structures | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 667636 | - |
dc.date.updated | 2017-02-16T11:54:22Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27705754 | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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667636.pdf | 1.07 MB | Adobe PDF | View/Open |
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