Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/107825
Title: | Production of highly bioactive resveratrol analogues pterostilbene and piceatannol in metabolically engineered grapevine cell cultures |
Author: | Martínez Márquez, Ascensión Morante Carriel, Jaime A. Ramírez Estrada, Karla Cusidó Vidal, Rosa M. Palazón Barandela, Javier Bru Martínez, Roque |
Keywords: | Cultiu cel·lular Metabolisme de les plantes Enginyeria genètica vegetal Vinyes Cell culture Plant metabolism Plant genetic engineering Vineyards |
Issue Date: | 11-Jan-2016 |
Publisher: | John Wiley & Sons |
Abstract: | Summary Grapevine stilbenes, particularly trans-resveratrol, have a demonstrated pharmacological activity. Other natural stilbenes derived from resveratrol such as pterostilbene or piceatannol, display higher oral bioavailability and bioactivity than the parent compound, but are far less abundant in natural sources. Thus, to efficiently obtain these bioactive resveratrol derivatives, there is a need to develop new bioproduction systems. Grapevine cell cultures are able to produce large amounts of easily recoverable extracellular resveratrol when elicited with methylated cyclodextrins and methyl jasmonate. We devised this system as an interesting starting point of a metabolic engineering-based strategy to produce resveratrol derivatives using resveratrolconverting enzymes. Constitutive expression of either Vitis vinifera resveratrol O-methyltransferase (VvROMT) or human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) led to pterostilbene or piceatannol, respectively, after the engineered cell cultures were treated with the aforementioned elicitors. Functionality of both gene products was first assessed in planta by Nicotiana benthamiana agroinfiltration assays, in which tobacco cells transiently expressed stilbene synthase and VvROMT or HsCYP1B1. Grapevine cell cultures transformed with VvROMT produced pterostilbene, which was detected in both intra- and extracellular compartments, at a level of micrograms per litre. Grapevine cell cultures transformed with HsCYP1B1 produced about 20 mg/L culture of piceatannol, displaying a sevenfold increase in relation to wild-type cultures, and reaching an extracellular distribution of up to 45% of total production. The results obtained demonstrate the feasibility of this novel system for the bioproduction of natural and more bioactive resveratrol derivatives and suggest new ways for the improvement of production yields |
Note: | Reproducció del document publicat a: https://doi.org/10.1111/pbi.12539 |
It is part of: | Plant Biotechnology Journal, 2016, vol. 14, p. 1813-1825 |
URI: | http://hdl.handle.net/2445/107825 |
Related resource: | https://doi.org/10.1111/pbi.12539 |
ISSN: | 1467-7644 |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
656817.pdf | 1.19 MB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License