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http://hdl.handle.net/2445/109604
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DC Field | Value | Language |
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dc.contributor.author | Jiménez, Alfons | - |
dc.contributor.author | Rees-Channer, Roxanne R. | - |
dc.contributor.author | Perera, Rushini | - |
dc.contributor.author | Gamboa, Dionicia | - |
dc.contributor.author | Chiodini, Peter L. | - |
dc.contributor.author | Gonzalez, Iveth J. | - |
dc.contributor.author | Mayor Aparicio, Alfredo Gabriel | - |
dc.contributor.author | Ding, Xavier C. | - |
dc.date.accessioned | 2017-04-11T07:23:17Z | - |
dc.date.available | 2017-04-11T07:23:17Z | - |
dc.date.issued | 2017-03-24 | - |
dc.identifier.issn | 1475-2875 | - |
dc.identifier.uri | http://hdl.handle.net/2445/109604 | - |
dc.description.abstract | BACKGROUND: Rapid diagnostic tests (RDTs) are today the most widely used method for malaria diagnosis and are recommended, alongside microscopy, for the confirmation of suspected cases before the administration of anti-malarial treatment. The diagnostic performance of RDTs, as compared to microscopy or PCR is well described but the actual analytical sensitivity of current best-in-class tests is poorly documented. This value is however a key performance indicator and a benchmark value needed to developed new RDTs of improved sensitivity. METHODS: Thirteen RDTs detecting either the Plasmodium falciparum histidine rich protein 2 (HRP2) or the plasmodial lactate dehydrogenase (pLDH) antigens were selected from the best performing RDTs according to the WHO-FIND product testing programme. The analytical sensitivity of these products was evaluated using a range of reference materials including P. falciparum and Plasmodium vivax whole parasite samples as well as recombinant proteins. RESULTS: The best performing HRP2-based RDTs could detect all P. falciparum cultured samples at concentrations as low as 0.8 ng/mL of HRP2. The limit of detection of the best performing pLDH-based RDT specifically detecting P. vivax was 25 ng/mL of pLDH. CONCLUSION: The analytical sensitivity of P. vivax and Pan pLDH-based RDTs appears to vary considerably from product to product, and improvement of the limit-of-detection for P. vivax detecting RDTs is needed to match the performance of HRP2 and Pf pLDH-based RDTs for P. falciparum. Different assays using different reference materials produce different values for antigen concentration in a given specimen, highlighting the need to establish universal reference assays. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1186/s12936-017-1780-5 | - |
dc.relation.ispartof | Malaria Journal, 2017, vol. 16, num. 128 | - |
dc.relation.uri | http://dx.doi.org/10.1186/s12936-017-1780-5 | - |
dc.rights | cc by (c) Jiménez et al., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Malària | - |
dc.subject.classification | Diagnòstic | - |
dc.subject.other | Malaria | - |
dc.subject.other | Diagnosis | - |
dc.title | Analytical sensitivity of current best-in-class malaria rapid diagnostic tests | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2017-04-05T18:01:24Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 28340585 | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
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jimenez2017_2483.pdf | 849.59 kB | Adobe PDF | View/Open |
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