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Title: | ERK5/BMK1 is a novel target of the tumor suppressor VHL: implication in clear cell renal carcinoma |
Author: | Arias-González, Laura Moreno-Gimeno, Inmaculada Rubio del Campo, Antonio Serrano-Oviedo, Leticia Valero, María Llanos Esparís-Ogando, Azucena Cruz-Morcillo, Miguel Ángel de la Melgar-Rojas, Pedro García-Cano, Jesús Cimas, Francisco J. Ruiz Hidalgo, María José Prado, Alfonso Callejas-Valera, Juan Luis Nam-Cha, Syong Hyun Giménez-Bachs, José Miguel Salinas-Sánchez, Antonio S. Pandiella, Atanasio Peso, Luis del Sánchez-Prieto, Ricardo |
Keywords: | Tumors Càncer de ronyó Antioncogens Proteïnes quinases Ubiqüitina Tumors Renal cancer Antioncogenes Protein kinases Ubiquitin |
Issue Date: | Jun-2013 |
Publisher: | Elsevier |
Abstract: | Extracellular signal-regulated kinase 5 (ERK5), also known as big mitogen-activated protein kinase (MAPK) 1, is implicated in a wide range of biologic processes, which include proliferation or vascularization. Here, we show that ERK5 is degraded through the ubiquitin-proteasome system, in a process mediated by the tumor suppressor von Hippel-Lindau (VHL) gene, through a prolyl hydroxylation-dependent mechanism. Our conclusions derive from transient transfection assays in Cos7 cells, as well as the study of endogenous ERK5 in different experimental systems such as MCF7, HMEC, or Caki-2 cell lines. In fact, the specific knockdown of ERK5 in pVHL-negative cell lines promotes a decrease in proliferation and migration, supporting the role of this MAPK in cellular transformation. Furthermore, in a short series of fresh samples from human clear cell renal cell carcinoma, high levels of ERK5 correlate with more aggressive and metastatic stages of the disease. Therefore, our results provide new biochemical data suggesting that ERK5 is a novel target of the tumor suppressor VHL, opening a new field of research on the role of ERK5 in renal carcinomas. |
Note: | Hi ha quatre pàgines de material suplementari sense numeració |
Note: | Reproducció del document publicat a: https://doi.org/10.1593/neo.121896 |
It is part of: | Neoplasia, 2013, vol. 15, num. 6, p. 649-659 |
URI: | http://hdl.handle.net/2445/110384 |
Related resource: | https://doi.org/10.1593/neo.121896 |
ISSN: | 1476-5586 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
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