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http://hdl.handle.net/2445/110939
Title: | 2-picolylamine derivatization for high sensitivity detection of abscisic acid in apicomplexan blood-infecting parasites |
Author: | Moles Meler, Ernest Marcos, Josep Imperial Ródenas, Santiago Pozo, Oscar J. Fernàndez Busquets, Xavier |
Keywords: | Malària Plasmodium falciparum Protozous patògens Àcid abscísic Malaria Plasmodium falciparum Pathogenic protozoa Abscisic acid |
Issue Date: | 11-Mar-2017 |
Publisher: | Elsevier Science Pub. Co. |
Abstract: | We have developed a new liquid chromatography-electrospray ionization tandem mass spectrometry methodology based on 2-picolylamine derivatization and positive ion mode detection for abscisic acid (ABA) identification. The selected reaction leads to the formation of an amide derivative which contains a highly active pyridyl group. The enhanced ionization allows for a 700-fold increase over commonly monitored unmodified ABA, which in turn leads to excellent limits of detection and quantification values of 0.03 and 0.15ngmL-1, respectively. This method has been validated in the highly complex matrix of a red blood cell extract. In spite of the high sensitivity achieved, ABA could not be detected in Plasmodium falciparum-infected red blood cells, suggesting that, if present, it will be found either in ultratrace amounts or as brief bursts at defined time points within the intraerythrocytic cycle and/or in the form of a biosynthetic analogue. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.talanta.2017.03.030 |
It is part of: | Talanta, 2017, vol. 168, p. 130-135 |
URI: | http://hdl.handle.net/2445/110939 |
Related resource: | http://dx.doi.org/10.1016/j.talanta.2017.03.030 |
ISSN: | 0039-9140 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
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