Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/111087
Title: | The chromatin remodeller CHD8 is required for E2F-dependent transcription activation of S-phase genes |
Author: | Subtil-Rodríguez, Alicia Vázquez-Chávez, Elena Ceballos-Chávez, María Rodríguez-Paredes, Manuel Martín-Subero, José Ignacio Esteller, Manel Reyes, José C. |
Keywords: | Cromatina Transcripció genètica Fixació de proteïnes Cicle cel·lular Proliferació cel·lular Histones Chromatin Genetic transcription Protein binding Cell cycle Cell proliferation Histones |
Issue Date: | Feb-2014 |
Publisher: | Oxford University Press |
Abstract: | The precise regulation of S-phase-specific genes is critical for cell proliferation. How the repressive chromatin configuration mediated by the retinoblastoma protein and repressor E2F factors changes at the G1/S transition to allow transcription activation is unclear. Here we show ChIP-on-chip studies that reveal that the chromatin remodeller CHD8 binds ∼2000 transcriptionally active promoters. The spectrum of CHD8 target genes was enriched in E2F-dependent genes. We found that CHD8 binds E2F-dependent promoters at the G1/S transition but not in quiescent cells. Consistently, CHD8 was required for G1/S-specific expression of these genes and for cell cycle re-entry on serum stimulation of quiescent cells. We also show that CHD8 interacts with E2F1 and, importantly, loading of E2F1 and E2F3, but not E2F4, onto S-specific promoters, requires CHD8. However, CHD8 recruiting is independent of these factors. Recruiting of MLL histone methyltransferase complexes to S-specific promoters was also severely impaired in the absence of CHD8. Furthermore, depletion of CHD8 abolished E2F1 overexpression-dependent S-phase stimulation of serum-starved cells, highlighting the essential role of CHD8 in E2F-dependent transcription activation. |
Note: | Reproducció del document publicat a: https://doi.org/10.1093/nar/gkt1161 |
It is part of: | Nucleic Acids Research, 2014, vol. 42, num. 4, p. 2185-2196 |
URI: | http://hdl.handle.net/2445/111087 |
Related resource: | https://doi.org/10.1093/nar/gkt1161 |
ISSN: | 0305-1048 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Fonaments Clínics) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
642636.pdf | 4.49 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License