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Title: Single-cell transcriptome conservation in cryopreserved cells and tissues
Author: Guillaumet Adkins, Amy
Rodríguez Esteban, Gustavo
Mereu, Elisabetta
Méndez Lago, María
Jaitin, Diego A.
Villanueva Garatachea, Alberto
Vidal-Bel, August
Martínez Martí, Alex
Felip, Enriqueta
Vivancos, Ana
Keren-Shaul, Hadas
Heath, Simon C.
Gut, Marta
Amit, Ido
Gut, Ivo G.
Heyn, Holger
Keywords: RNA
Crioconservació d'òrgans
Transcripció genètica
Cryopreservation of organs
Genetic transcription
Issue Date: 1-Mar-2017
Publisher: BioMed Central
Abstract: A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.
Note: Reproducció del document publicat a:
It is part of: Genome Biology, 2017, vol. 18, p. 45
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ISSN: 1474-7596
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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