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Title: | A Novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea |
Author: | Campillo, Noelia Jorba, Ignasi Schaedel, Laura Casals, Blai Gozal, David Farré Ventura, Ramon Almendros López, Isaac Navajas Navarro, Daniel |
Keywords: | Síndromes d'apnea del son Oxigen en l'organisme Respiració Sleep apnea syndromes Oxygen in the body Respiration |
Issue Date: | 29-Jul-2016 |
Publisher: | Frontiers Media |
Abstract: | Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), plays a critical role in the pathogenesis of OSA-associated morbidities, especially in the cardiovascular and respiratory systems. Oxidative stress and inflammation induced by IH are suggested as main contributors of end-organ dysfunction in OSA patients and animal models. Since the molecular mechanisms underlying these in vivo pathological responses remain poorly understood, implementation of experimental in vitro cell-based systems capable of inducing high-frequency IH would be highly desirable. Here, we describe the design, fabrication, and validation of a versatile chip for subjecting cultured cells to fast changes in gas partial pressure and to cyclic stretch. The chip is fabricated with polydimethylsiloxane (PDMS) and consists of a cylindrical well-covered by a thin membrane. Cells cultured on top of the membrane can be subjected to fast changes in oxygen concentration (equilibrium time similar to 6 s). Moreover, cells can be subjected to cyclic stretch at cardiac or respiratory frequencies independently or simultaneously. Rat bone marrow-derived mesenchymal stem cells (MSCs) exposed to IH mimicking OSA and cyclic stretch at cardiac frequencies revealed that hypoxia-inducible factor 1 alpha (HIF-1 alpha) expression was increased in response to both stimuli. Thus, the chip provides a versatile tool for the study of cellular responses to cyclical hypoxia and stretch. |
Note: | Reproducció del document publicat a: https://doi.org/10.3339/fphys.2016.00319 |
It is part of: | Frontiers in Physiology, 2016, vol. 7, num. 319 |
URI: | http://hdl.handle.net/2445/112377 |
Related resource: | https://doi.org/10.3339/fphys.2016.00319 |
ISSN: | 1664-042X |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
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