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http://hdl.handle.net/2445/113724
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DC Field | Value | Language |
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dc.contributor.author | Barriga, Francisco M. | - |
dc.contributor.author | Montagni, Elisa | - |
dc.contributor.author | Mana, Miyeko | - |
dc.contributor.author | Méndez Lago, María | - |
dc.contributor.author | Hernando Momblona, Xavier | - |
dc.contributor.author | Sevillano, Marta | - |
dc.contributor.author | Guillaumet Adkins, Amy | - |
dc.contributor.author | Rodríguez Esteban, Gustavo | - |
dc.contributor.author | Buczacki, Simon J. A. | - |
dc.contributor.author | Gut, Marta | - |
dc.contributor.author | Heyn, Holger | - |
dc.contributor.author | Winton, Douglas J. | - |
dc.contributor.author | Yilmaz, Omer H. | - |
dc.contributor.author | Stephan-Otto Attolini, Camille | - |
dc.contributor.author | Gut, Ivo G. | - |
dc.contributor.author | Batlle, Eduard | - |
dc.date.accessioned | 2017-07-13T08:08:26Z | - |
dc.date.available | 2018-03-09T23:01:22Z | - |
dc.date.issued | 2017-03-09 | - |
dc.identifier.issn | 1934-5909 | - |
dc.identifier.uri | http://hdl.handle.net/2445/113724 | - |
dc.description.abstract | Highly proliferative Lgr5+ stem cells maintain the intestinal epithelium and are thought to be largely homogeneous. Although quiescent intestinal stem cell (ISC) populations have been described, the identity and features of such a population remain controversial. Here we report unanticipated heterogeneity within the Lgr5+ ISC pool. We found that expression of the RNA-binding protein Mex3a labels a slowly cycling subpopulation of Lgr5+ ISCs that contribute to all intestinal lineages with distinct kinetics. Single-cell transcriptome profiling revealed that Lgr5+ cells adopt two discrete states, one of which is defined by a Mex3a expression program and relatively low levels of proliferation genes. During homeostasis, Mex3a+ cells continually shift into the rapidly dividing, self-renewing ISC pool. Chemotherapy and radiation preferentially target rapidly dividing Lgr5+ cells but spare the Mex3a-high/Lgr5+ population, helping to promote regeneration of the intestinal epithelium following toxic insults. Thus, Mex3a defines a reserve-like ISC population within the Lgr5+ compartment. | eng |
dc.format.extent | 62 p. | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.stem.2017.02.007 | - |
dc.relation.ispartof | Cell Stem Cell, 2017 | - |
dc.relation.uri | http://dx.doi.org/10.1016/j.stem.2017.02.007 | - |
dc.rights | cc by-nc-nd (c) Elsevier, 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | - |
dc.source | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) | - |
dc.subject.classification | Cèl·lules mare | - |
dc.subject.classification | RNA | - |
dc.subject.other | Stem cells | - |
dc.subject.other | RNA | - |
dc.title | Mex3a Marks a Slowly Dividing Subpopulation of Lgr5+ Intestinal Stem Cells | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.date.updated | 2017-07-13T08:07:16Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/340176/EU//EDITCRC | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
This item is licensed under a Creative Commons License