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http://hdl.handle.net/2445/114446
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DC Field | Value | Language |
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dc.contributor.author | Jin, Yi | - |
dc.contributor.author | Petricevic, Marija | - |
dc.contributor.author | John, Alan | - |
dc.contributor.author | Raich Armendáriz, Lluís Adrià | - |
dc.contributor.author | Jenkins, Huw | - |
dc.contributor.author | Portela de Souza, Leticia | - |
dc.contributor.author | Cuskin, Fiona | - |
dc.contributor.author | Gilbert, Harry J. | - |
dc.contributor.author | Rovira i Virgili, Carme | - |
dc.contributor.author | Goddard-Borger, Ethan D. | - |
dc.contributor.author | Williams, Spencer J. | - |
dc.contributor.author | Davies, Gideon J. | - |
dc.date.accessioned | 2017-07-27T10:53:12Z | - |
dc.date.available | 2017-07-27T10:53:12Z | - |
dc.date.issued | 2016-11-08 | - |
dc.identifier.issn | 2374-7951 | - |
dc.identifier.uri | http://hdl.handle.net/2445/114446 | - |
dc.description.abstract | The enzymatic cleavage of β-1,4-mannans is achieved by endo-β-1,4-mannanases, enzymes involved in germination of seeds and microbial hemicellulose degradation, and which have increasing industrial and consumer product applications. β-Mannanases occur in a range of families of the CAZy sequence-based glycoside hydrolase (GH) classification scheme including families 5, 26, and 113. In this work we reveal that β-mannanases of the newly described GH family 134 differ from other mannanase families in both their mechanism and tertiary structure. A representative GH family 134 endo-β-1,4-mannanase from a Streptomyces sp. displays a fold closely related to that of hen egg white lysozyme but acts with inversion of stereochemistry. A Michaelis complex with mannopentaose, and a product complex with mannotriose, reveal ligands with pyranose rings distorted in an unusual inverted chair conformation. Ab initio quantum mechanics/molecular mechanics metadynamics quantified the energetically accessible ring conformations and provided evidence in support of a 1C4 → 3H4 → 3S1 conformational itinerary along the reaction coordinate. This work, in concert with that on GH family 124 cellulases, reveals how the lysozyme fold can be co-opted to catalyze the hydrolysis of different polysaccharides in a mechanistically distinct manner | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1021/acscentsci.6b00232 | - |
dc.relation.ispartof | ACS Central Science, 2016, vol. 2, num. 12, p. 896-903 | - |
dc.relation.uri | https://doi.org/10.1021/acscentsci.6b00232 | - |
dc.rights | (c) American Chemical Society , 2016 | - |
dc.source | Articles publicats en revistes (Química Inorgànica i Orgànica) | - |
dc.subject.classification | Lisozim | - |
dc.subject.classification | Enzims | - |
dc.subject.classification | Catàlisi | - |
dc.subject.other | Lysozyme | - |
dc.subject.other | Enzymes | - |
dc.subject.other | Catalysis | - |
dc.title | A β-mannannase with a lysozyme fold and a novel molecular catalytic mechanism | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 667980 | - |
dc.date.updated | 2017-07-27T10:53:12Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/322942/EU//GLYCOPOISE | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 28058278 | - |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) |
Files in This Item:
File | Description | Size | Format | |
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667980.pdf | 586.62 kB | Adobe PDF | View/Open |
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