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Title: | Biophysical characterization of the association of histones with single-stranded DNA |
Author: | Wang, Ying Merwyk, Luis Van Tönsing, Katja Walhorn, Volker Anselmetti, Dario Fernàndez Busquets, Xavier |
Keywords: | Histones Cromatina Histones Chromatine |
Issue Date: | 27-Jul-2017 |
Publisher: | Elsevier |
Abstract: | BACKGROUND: Despite the profound current knowledge of the architecture and dynamics of nucleosomes, little is known about the structures generated by the interaction of histones with single-stranded DNA (ssDNA), which is widely present during replication and transcription. METHODS: Non-denaturing gel electrophoresis, transmission electron microscopy, atomic force microscopy, magnetic tweezers. RESULTS: Histones have a high affinity for ssDNA at physiological salt concentrations, with an apparent binding constant similar to that calculated for their association with double-stranded DNA (dsDNA). The length of DNA (number of nucleotides in ssDNA or base pairs in dsDNA) associated with a fixed core histone mass is the same for both ssDNA and dsDNA. Whereas histone-ssDNA complexes show a high tendency to aggregate in 0.2 M NaCl, at lower ionic strength nucleosome-like structures are formed. Core histones are able to protect ssDNA from digestion by micrococcal nuclease, and a shortening of ssDNA occurs upon its interaction with histones. The purified (+) strand of a cloned DNA fragment of nucleosomal origin has a higher affinity for histones than the purified complementary (-) strand. CONCLUSIONS: At physiological ionic strength histones have high affinity for ssDNA, possibly associating with it into nucleosome-like structures. General Significance In the cell nucleus histones may spontaneously interact with ssDNA to facilitate their participation in the replication and transcription of chromatin. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.bbagen.2017.07.018 |
It is part of: | Biochimica et Biophysica Acta. General Subjects, 2017 |
URI: | http://hdl.handle.net/2445/115793 |
Related resource: | http://dx.doi.org/10.1016/j.bbagen.2017.07.018 |
ISSN: | 0006-3002 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
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