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http://hdl.handle.net/2445/119377
Title: | miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation |
Author: | Viñas Muñoz, José Luis Ventayol Espinazo, Marina Brüne, Bernhard, 1957- Jung, Michaela Sola Martínez, Anna Pi i Siqués, Felip Mastora, Chrysoula Hotter Corripio, Georgina |
Keywords: | Cèl·lules mare embrionàries Micro RNAs Diferenciació cel·lular Embryonic stem cells MicroRNAs Cell diferentiation |
Issue Date: | 10-Apr-2013 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0060937 |
It is part of: | PLoS One, 2013, vol. 8, num. 4, p. e60937 |
URI: | http://hdl.handle.net/2445/119377 |
Related resource: | https://doi.org/10.1371/journal.pone.0060937 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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