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Title: Discovery and validation of new potential biomarkers for early detection of colon cancer
Author: Solé Acha, Xavier
Crous Bou, Marta
Cordero Romera, David
Olivares, David
Guinó, Elisabet
Sanz Pamplona, Rebeca
Rodríguez Moranta, Francisco
Sanjuan, Xavier
Oca Burguete, Javier de
Salazar Soler, Ramón
Moreno Aguado, Víctor
Keywords: Marcadors tumorals
Marcadors bioquímics
Càncer colorectal
Tumor markers
Biochemical markers
Colorectal cancer
Issue Date: 12-Sep-2014
Publisher: Public Library of Science (PLoS)
Abstract: Background: accurate detection of characteristic proteins secreted by colon cancer tumor cells in biological fluids could serve as a biomarker for the disease. The aim of the present study was to identify and validate new serum biomarkers and demonstrate their potential usefulness for early diagnosis of colon cancer. Methods: the study was organized in three sequential phases: 1) biomarker discovery, 2) technical and biological validation, and 3) proof of concept to test the potential clinical use of selected biomarkers. A prioritized subset of the differentially-expressed genes between tissue types (50 colon mucosa from cancer-free individuals and 100 normal-tumor pairs from colon cancer patients) was validated and further tested in a series of serum samples from 80 colon cancer cases, 23 patients with adenoma and 77 cancer-free controls.Results: in the discovery phase, 505 unique candidate biomarkers were identified, with highly significant results and high capacity to discriminate between the different tissue types. After a subsequent prioritization, all tested genes (N = 23) were successfully validated in tissue, and one of them, COL10A1, showed relevant differences in serum protein levels between controls, patients with adenoma (p = 0.0083) and colon cancer cases (p = 3.2e-6). Conclusion: we present a sequential process for the identification and further validation of biomarkers for early detection of colon cancer that identifies COL10A1 protein levels in serum as a potential diagnostic candidate to detect both adenoma lesions and tumor. Impact:the use of a cheap serum test for colon cancer screening should improve its participation rates and contribute to decrease the burden of this disease.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2014, vol. 9, num. 9, p. e106748
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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