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http://hdl.handle.net/2445/119785
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DC Field | Value | Language |
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dc.contributor.author | Benito, S. | - |
dc.contributor.author | Ferrer, Assumpta | - |
dc.contributor.author | Benabou Zdaou, Sanae | - |
dc.contributor.author | Aviñó Andrés, Anna | - |
dc.contributor.author | Eritja i Casadellà, Ramon | - |
dc.contributor.author | Gargallo Gómez, Raimundo | - |
dc.date.accessioned | 2018-02-13T10:42:47Z | - |
dc.date.available | 2020-02-06T06:10:13Z | - |
dc.date.issued | 2018-02-06 | - |
dc.identifier.issn | 1386-1425 | - |
dc.identifier.uri | http://hdl.handle.net/2445/119785 | - |
dc.description.abstract | Guanine-rich sequences may fold into highly ordered structures known as G-quadruplexes. Apart from the monomeric G-quadruplex, these sequences may form multimeric structures that are not usually considered when studying interaction with ligands. This work studies the interaction of a ligand, crystal violet, with three guanine-rich DNA sequences with the capacity to form multimeric structures. These sequences correspond to short stretches found near the promoter regions of c-kit and SMARCA4 genes. Instrumental techniques (circular dichroism, molecular fluorescence, size-exclusion chromatography and electrospray ionization mass spectrometry) and multivariate data analysis were used for this purpose. The polymorphism of G-quadruplexes was characterized prior to the interaction studies. The ligand was shown to interact preferentially with the monomeric G-quadruplex; the binding stoichiometry was 1:1 and the binding constant was in the order of 105 M-1 for all three sequences. The results highlight the importance of DNA treatment prior to interaction studies | - |
dc.format.extent | 42 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.saa.2018.02.006 | - |
dc.relation.ispartof | Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 2018 | - |
dc.relation.uri | https://doi.org/10.1016/j.saa.2018.02.006 | - |
dc.rights | cc-by-nc-nd (c) Elsevier B.V., 2018 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Polimorfisme (Cristal·lografia) | - |
dc.subject.classification | Anàlisi multivariable | - |
dc.subject.classification | Lligands | - |
dc.subject.classification | G-estructures | - |
dc.subject.other | Polymorphism (Crystallography) | - |
dc.subject.other | Multivariate analysis | - |
dc.subject.other | Ligands | - |
dc.subject.other | G-structures | - |
dc.title | Evaluation of the effect of polymorphism on G-quadruplex-ligand interaction by means of spectroscopic and chromatographic techniques | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 676132 | - |
dc.date.updated | 2018-02-13T10:42:47Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 29448171 | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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File | Description | Size | Format | |
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676132.pdf | 2.32 MB | Adobe PDF | View/Open |
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