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Title: | New biochemical insights into the mechanisms of pulmonary arterial hypertension in humans |
Author: | Bujak, Renata Mateo, Jesús Blanco Vich, Isabel Izquierdo-García, José Luis Dudzik, Danuta Markuszewski, Michal J. Peinado Cabré, Víctor Ivo Laclaustra, Martín Barberà i Mir, Joan Albert Barbas, Coral Ruiz-Cabello, Jesús |
Keywords: | Hipertensió pulmonar Cromatografia de líquids Espectrofotometria Plasma sanguini Metabòlits Glucòlisi Ionització Pulmonary hypertension Liquid chromatography Spectrophotometry Blood plasma Metabolites Glycolysis Ionization |
Issue Date: | 3-Aug-2016 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Diagnosis of pulmonary arterial hypertension (PAH) is difficult due to the lack of specific clinical symptoms and biomarkers, especially at early stages. We compared plasma metabolic fingerprints of PAH patients (n = 20) with matched healthy volunteers (n = 20) using, for the first time, untargeted multiplatform metabolomics approach consisting of high-performance liquid and gas chromatography coupled with mass spectrometry. Multivariate statistical analyses were performed to select metabolites that contribute most to groups' classification (21 from liquid in both ionization modes and 9 from gas chromatography-mass spectrometry). We found metabolites related to energy imbalance, such as glycolysis-derived metabolites, as well as metabolites involved in fatty acid, lipid and amino acid metabolism. We observed statistically significant changes in threitol and aminomalonic acid in PAH patients, which could provide new biochemical insights into the pathogenesis of the disease. The results were externally validated on independent case and control cohorts, confirming up to 16 metabolites as statistically significant in the validation study. Multiplatform metabolomics, followed by multivariate chemometric data analysis has a huge potential for explaining pathogenesis of PAH and for searching potential and new more specific and less invasive markers of the disease. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0160505 |
It is part of: | PLoS One, 2016, vol. 11, p. e0160505 |
URI: | http://hdl.handle.net/2445/119963 |
Related resource: | https://doi.org/10.1371/journal.pone.0160505 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Medicina) |
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