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Title: Filamin B plays a key role in VEGF-induced endothelial cell motility through its interaction with Rac-1 and Vav-2
Author: Del Valle-Pérez, Beatriz
Martinez, Vanesa Gabriela
Lacasa Salavert, Cristina
Figueras i Amat, Agnès
Shapiro, Sandor S.
Takafuta, Toshiro
Casanovas i Casanovas, Oriol
Capellá, G. (Gabriel)
Ventura Pujol, Francesc
Viñals Canals, Francesc
Keywords: Proteïnes citosquelètiques
Migració cel·lular
Cytoskeletal proteins
Cell migration
Issue Date: 2-Apr-2010
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: Actin-binding proteins filamin A (FLNA) and B (FLNB) are expressed in endothelial cells and play an essential role during vascular development. In order to investigate their role in adult endothelial cell function, we initially confirmed their expression pattern in different adult mouse tissues and cultured cell lines and found that FLNB expression is concentrated mainly in endothelial cells while FLNA is more ubiquitously expressed. Functionally, siRNA-knockdown of endogenous FLNB in HUVEC inhibited Vascular Endothelial Growth Factor (VEGF)-induced in vitro angiogenesis by decreasing endothelial cell migration capacity, whereas FLNA ablation did not alter these parameters. Moreover, FLNB-depleted cells increased their substrate adhesion with more focal adhesions. The molecular mechanism underlying this effect implicates modulation of small GTP binding protein Rac-1 localization and activity, with altered activation of its downstream effectors p21 protein Cdc42/Rac-activated kinase (PAK)-4/5/6 and its activating guanine nucleotide exchange factor Vav-2. Moreover, our results suggest the existence of a signaling complex including FLNB, Rac-1 and Vav-2 under basal conditions that would further interact with VEGFR2 and integrin αVβ5 after VEGF stimulation. In conclusion, our results reveal a crucial role for FLNB in endothelial cell migration and in the angiogenic process in adult endothelial cells.
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It is part of: Journal of Biological Chemistry, 2010, vol. 285, num. 14, p. 10748-10760
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ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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