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http://hdl.handle.net/2445/120682
Title: | Endocytic reawakening of motility in jammed epithelia. |
Author: | Malinverno, Chiara Corallino, Salvatore Giavazzi, Fabio Bergert, Martin Li, Qingsen Leoni, Marco Disanza, Andrea Frittoli, Emanuela Oldani, Amanda Martini, Emanuele Lendenmann, Tobias Deflorian, Gianluca Beznoussenko, Galina V. Poulikakos, Dimos ONG Kok Haur Uroz, Marina Trepat Guixer, Xavier Parazzoli, Dario Maiuri, Paolo Yu, Weimiao Ferrari, Aldo Cerbino, Roberto Scita, Giorgio |
Keywords: | Epiteli Proteïnes Motilitat cel·lular Epithelium Proteins Cell motility |
Issue Date: | 1-May-2017 |
Publisher: | Nature Publishing Group |
Abstract: | Dynamics of epithelial monolayers has recently been interpreted in terms of a jamming or rigidity transition. How cells control such phase transitions is, however, unknown. Here we show that RAB5A, a key endocytic protein, is sufficient to induce large-scale, coordinated motility over tens of cells, and ballistic motion in otherwise kinetically arrested monolayers. This is linked to increased traction forces and to the extension of cell protrusions, which align with local velocity. Molecularly, impairing endocytosis, macropinocytosis or increasing fluid efflux abrogates RAB5A-induced collective motility. A simple model based on mechanical junctional tension and an active cell reorientation mechanism for the velocity of self-propelled cells identifies regimes of monolayer dynamics that explain endocytic reawakening of locomotion in terms of a combination of large-scale directed migration and local unjamming. These changes in multicellular dynamics enable collectives to migrate under physical constraints and may be exploited by tumours for interstitial dissemination. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1038/nmat4848 |
It is part of: | Nature Materials, 2017, vol. 16, num. 5, p. 587-596 |
URI: | http://hdl.handle.net/2445/120682 |
Related resource: | https://doi.org/10.1038/nmat4848 |
ISSN: | 1476-1122 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Publicacions de projectes de recerca finançats per la UE Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
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