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Title: Genetic diversity in Silene sennenii Pau (Caryophyllaceae) assayed through DNA-based techniques
Author: López Viñallonga, Sara
López i Pujol, Jordi
Martinell Andreu, Maria del Carmen
Massó i Alemán, Sergi
Blanché i Vergés, Cèsar
Keywords: Plantes endèmiques
Conservació de la diversitat biològica
Endemic plants
Biodiversity conservation
Issue Date: 2012
Publisher: Consejo Superior de Investigaciones Científicas (CSIC)
Abstract: Genetic diversity in Silene sennenii Pau (Caryophyllaceae) assayed through DNA-based techniques. Silene sennenii is a narrow endemic species from the NE of the Iberian Peninsula. It is considered as EN ("Endangered") according to the IUCN criteria and is under legal protection in Catalonia. In the present work we report an assay using three different approaches for surveying the genetic diversity in this rare, endangered campion: analysis of chloroplast haplotypes, AFLPs and transferability of microsatellites previously developed for Silene latifolia. None of the nine chloroplast regions sequenced showed any variability. Five out of the 21 microsatellite loci tested were polymorphic although more loci are required in order to perform a robust population genetics study. Regarding the AFLP analysis, five out of the 26 pairs of primers tested exhibited moderate levels of variability and therefore they could be useful for further investigating the genetic structure of S. sennenii. Although preliminary, our results based on three different genetic markers are in agreement with the low values of genetic variation already reported for this species employing allozymes.
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It is part of: Collectanea Botanica, 2012, vol. 31, p. 7-18
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ISSN: 0010-0730
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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