Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/121061
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Vázquez de la Torre Cervera, Aurelio | - |
dc.contributor.author | Gay i Marín, Marina | - |
dc.contributor.author | Vilaprinyó Pascual, Sílvia | - |
dc.contributor.author | Mazzucato, Roberta | - |
dc.contributor.author | Serra Batiste, Montserrat | - |
dc.contributor.author | Vilaseca Casas, Marta | - |
dc.contributor.author | Carulla Casanovas, Natàlia | - |
dc.date.accessioned | 2018-03-23T11:21:16Z | - |
dc.date.available | 2019-03-14T06:10:21Z | - |
dc.date.issued | 2018-03-14 | - |
dc.identifier.uri | http://hdl.handle.net/2445/121061 | - |
dc.description.abstract | Brain-derived amyloid-β (Aβ) dimers are associated with Alzheimer´s disease (AD). However, their covalent nature remains controversial. This feature is relevant, as a covalent cross-link would make brain-derived dimers (brain dimers) more synaptotoxic than Aβ monomers and would make them suitable candidates for biomarker development. To resolve this controversy, we here present a three-step approach. First, we validated a type of synthetic cross-linked Aβ (CL Aβ) dimers, obtained by means of the photo-induced cross-linking of unmodified proteins (PICUP) reaction, as well-defined mimics of putative brain CL Aβ dimers. Second, we used these PICUP CL Aβ dimers as standards to improve the isolation of brain Aβ dimers and to develop state-of-the-art mass spectrometry (MS) strategies to allow their characterization. Third, we applied these MS methods to the analysis of brain Aβ dimer samples allowing the detection of the CL [Aβ(6-16)]2 peptide comprising a dityrosine cross-link. This result demonstrates the presence of CL Aβ dimers in the brains of patients with AD and opens up avenues for establishing new therapeutic targets and developing novel biomarkers for this disease. | - |
dc.format.extent | 31 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1021/acs.analchem.7b04936 | - |
dc.relation.ispartof | Analytical Chemistry, 2018 | - |
dc.relation.uri | http://dx.doi.org/10.1021/acs.analchem.7b04936 | - |
dc.rights | (c) American Chemical Society, 2018 | - |
dc.source | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) | - |
dc.subject.classification | Malaltia d'Alzheimer | - |
dc.subject.classification | Marcadors bioquímics | - |
dc.subject.other | Alzheimer's disease | - |
dc.subject.other | Biochemical markers | - |
dc.title | Direct evidence of the presence of cross-linked Aβ dimers in the brains of Alzheimer's disease patients | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.date.updated | 2018-03-20T11:59:58Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
1803_Ab_Native_CL_D_Anal_Chem.pdf | 3.13 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.