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DC Field | Value | Language |
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dc.contributor.author | Lizana, X. | - |
dc.contributor.author | López Sevilla, Asunción | - |
dc.contributor.author | Benito, Silvia | - |
dc.contributor.author | Agustí, Gemma | - |
dc.contributor.author | Ríos Alcolea, Martín | - |
dc.contributor.author | Piqué i Clusella, Núria | - |
dc.contributor.author | Marqués Villavecchia, Ana M. | - |
dc.contributor.author | Codony, Francesc | - |
dc.date.accessioned | 2018-04-18T13:09:01Z | - |
dc.date.available | 2018-12-31T06:10:22Z | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 1438-4639 | - |
dc.identifier.uri | http://hdl.handle.net/2445/121682 | - |
dc.description.abstract | Background Viability quantitative Polymerase Chain Reaction (v-qPCR) is a recent analytical approach for only detecting live microorganisms by DNA amplification-based methods This approach is based on the use of a reagent that irreversibly fixes dead cells DNA. In this study, we evaluate the utility of v-qPCR versus culture method for Legionellosis risk management. Methods The present study was performed using 116 real samples. Water samples were simultaneously analysed by culture, v-qPCR and qPCR methods. Results were compared by means of a non-parametric test. Results In 11.6% of samples using both methods (culture method and v-qPCR) results were positive, in 50.0% of samples both methods gave rise to negative results. As expected, equivalence between methods was not observed in all cases, as in 32.1% of samples positive results were obtained by v-qPCR and all of them gave rise to negative results by culture. Only in 6.3% of samples, with very low Legionella levels, was culture positive and v-qPCR negative. In 3.5% of samples, overgrowth of other bacteria did not allow performing the culture. When comparing both methods, significant differences between culture and v-qPCR were in the samples belonging to the cooling towers-evaporative condensers group. The v-qPCR method detected greater presence and obtained higher concentrations of Legionella spp. (p < 0.001). Otherwise, no significant differences between methods were found in the rest of the groups. Conclusions The v-qPCR method can be used as a quick tool to evaluate Legionellosis risk, especially in cooling towers-evaporative condensers, where this technique can detect higher levels than culture. The combined interpretation of PCR results along with the ratio of live cells is proposed as a tool for understanding the sample context and estimating the Legionellosis risk potential according to 4 levels of hierarchy. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.ijheh.2017.08.007 | - |
dc.relation.ispartof | International Journal of Hygiene and Environmental Health, 2017, vol. 220, num. 8, p. 1318-1324 | - |
dc.relation.uri | https://doi.org/10.1016/j.ijheh.2017.08.007 | - |
dc.rights | cc-by-nc-nd (c) Urban and Fischer, 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) | - |
dc.subject.classification | Legionel·la | - |
dc.subject.classification | Reacció en cadena de la polimerasa | - |
dc.subject.classification | ADN | - |
dc.subject.classification | Bacteris | - |
dc.subject.classification | Anàlisi de l'aigua | - |
dc.subject.other | Legionella | - |
dc.subject.other | Polymerase chain reaction | - |
dc.subject.other | DNA | - |
dc.subject.other | Bacteria | - |
dc.subject.other | Water analysis | - |
dc.title | Viability qPCR, a new tool for Legionella risk management | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 675570 | - |
dc.date.updated | 2018-04-18T13:09:02Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 28882518 | - |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
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pagination_IJHEH_13120.pdf | 707.03 kB | Adobe PDF | View/Open |
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