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http://hdl.handle.net/2445/121708
Title: | A semiquantitative scoring tool to evaluate eccentric exercise-induced muscle damage in trained rats |
Author: | Rizo Roca, David Ríos Kristjánsson, Juan Gabriel Núñez Espinosa, Cristian Ascensão, António Magalhães, José Torrella Guio, Joan Ramon Pagés, Teresa Viscor Carrasco, Ginés |
Keywords: | Malalties musculars Rates (Animals de laboratori) Exercici Muscular Diseases Rats as laboratory animals Exercise |
Issue Date: | 2-Nov-2015 |
Publisher: | PAGEPress |
Abstract: | Unaccustomed eccentric exercise is a welldocumented cause of exercise-induced muscle damage. However, in trained subjects muscle injury involves only light or moderate tissue damage. Since trained rats are widely used as a model for skeletal muscle injury, here we propose a semiquantitative scoring tool to evaluate muscle damage in trained rats. Twenty male Sprague-Dawley rats were trained for two weeks following a two-week preconditioning period, and randomly divided into two groups: control rats (CTL; n=5) and rats with eccentric exercise-induced muscle damage (INJ; n=15). Injured rats were sacrificed at three time points: 1, 3 and 7 days post injury (n=5 each). Transverse sections from the right soleus were cut (10 μm) and stained with haematoxylineosin. Samples were evaluated by two groups of observers (four researchers experienced in skeletal muscle histopathology and four inexperienced) using the proposed tool, which consisted of six items organised in three domains: abnormal fibre morphology, necrotic/(re) degenerating fibres (muscle fibre domain), endomysial and perimysial infiltration (inflammatory state domain) and endomysium and perimysium distension (interstitial compartment domain). We observed the expected time course in the six evaluated items. Furthermore, agreement among observers was evaluated by measuring the Intraclass Correlation Coefficient (ICC). Within the experienced group, items from the muscle fibre and interstitial compartment domains showed good agreement and the two items from the infiltration compartment domain showed excellent agreement. In conclusion, the proposed tool allowed quick and correct evaluation of light to moderate muscle damage in trained rats with good agreement between observers. |
Note: | Reproducció del document publicat a: https://doi.org/10.4081/ejh.2015.2544 |
It is part of: | European Journal of Histochemistry, 2015, vol. 59, num. 4, p. 2544 |
URI: | http://hdl.handle.net/2445/121708 |
Related resource: | https://doi.org/10.4081/ejh.2015.2544 |
ISSN: | 1121-760X |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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