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Title: Multiple Kv1.5 targeting to membrane surface microdomains
Author: Martínez Mármol, Ramón
Villalonga, Núria
Solé i Codina, Laura
Vicente García, Rubén, 1978-
Tamkun, Michael M.
Soler Prat, Concepció
Felipe Campo, Antonio
Keywords: Membranes cel·lulars
Cell membranes
Issue Date: 30-Jul-2008
Publisher: Wiley
Abstract: Surface expression of voltage-dependent K+ channels (Kv) has a pivotal role in leukocyte physiology. Although little is known about the physiological role of lipid rafts, these microdomains concentrate signaling molecules and their ion channel substrates. Kv1.3 associates with Kv1.5 to form functional channels in macrophages. Different isoform stoichiometries lead to distinct heteromeric channels which may be further modulated by targeting the complex to different membrane surface microdomains. Kv1.3 targets to lipid rafts, whereas Kv1.5 localization is under debate. With this in mind, we wanted to study whether heterotetrameric Kv1.5-containing channels target to lipid rafts. While in transfected HEK-293 cells, homo- and heterotetrameric channels targeted to rafts, Kv1.5 did not target to rafts in macrophages. Therefore, Kv1.3/Kv1.5 hybrid channels are mostly concentrated in non-raft microdomains. However, LPS-induced activation, which increases the Kv1.3/Kv1.5 ratio and caveolin, targeted Kv1.5 back to lipid rafts. Moreover, Kv1.5 did not localize to low-buoyancy fractions in L6E9 skeletal myoblasts, which also coexpress both channels, heart membranes or cardiomyocyes. Coexpression of a Cav3DGVmutant confined Kv1.5 to Cav3DGV-vesicles of HEK cells. Contrarily, coexpression of Kvβ2.1 impaired the Kv1.5 targeting to raft microdomains in HEK cells. Our results indicate that Kv1.5 partnership interactions are underlying mechanisms governing channel targeting to lipid rafts.
Note: Versió postprint del document publicat a:
It is part of: Journal of Cellular Physiology, 2008, vol. 217, num. 3, p. 667-673
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ISSN: 0021-9541
Appears in Collections:Articles publicats en revistes (Institut de Biomedicina (IBUB))
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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