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http://hdl.handle.net/2445/123700
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DC Field | Value | Language |
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dc.contributor.author | Carballar, Reyes | - |
dc.contributor.author | Canyelles, Maria de Lluc | - |
dc.contributor.author | Fernández, Claudia | - |
dc.contributor.author | Martí, Yasmina | - |
dc.contributor.author | Bonnin, Sarah | - |
dc.contributor.author | Castaño Boldú, Esther | - |
dc.contributor.author | Montanya Mias, Eduard | - |
dc.contributor.author | Téllez i Besolí, Noèlia | - |
dc.date.accessioned | 2018-07-17T10:23:02Z | - |
dc.date.available | 2018-07-17T10:23:02Z | - |
dc.date.issued | 2017-12-13 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2445/123700 | - |
dc.description.abstract | β-cell proliferation is a rare event in adult pancreatic islets. To study the replication-related β-cell biology we designed a replicating β-cells sorting system for gene expression experiments. Replicating β-cells were identifed by EdU incorporation and purifed by fow cytometry. For β-cell separation islet cells were sorted by size, granularity and Newport Green fuorescence emission that was combined with emitted fuorescence for EdU-labelled replicating cells sorting. The purity of the resulting sorted populations was evaluated by insulin staining and EdU for β-cell identifcation and for replicating cells, respectively. Total RNA was isolated from purifed cell-sorted populations for gene expression analysis. Cell sorting of dispersed islet cells resulted in 96.2% purity for insulin positivity in the collected β-cell fraction and 100% efciency of the EdU-based cell separation. RNA integrity was similar between FACSsorted replicating and quiescent β-cells. Global transcriptome analysis of replicating vs quiescent β-cells showed the expected enrichment of categories related to cell division and DNA replication. Indeed, key genes in the spindle check-point were the most upregulated genes in replicating β-cells. This work provides a method that allows for the isolation of replicating β-cells, a very scarce population in adult pancreatic islets. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41598-017-17776-2 | - |
dc.relation.ispartof | Scientific Reports, 2017, vol. 7, p. 17515 | - |
dc.relation.uri | https://doi.org/10.1038/s41598-017-17776-2 | - |
dc.rights | cc-by (c) Carballar, Reyes et al., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.classification | Malalties del pàncrees | - |
dc.subject.classification | Diabetis | - |
dc.subject.other | Gene expression | - |
dc.subject.other | Pancréas diseases | - |
dc.subject.other | Diabetes | - |
dc.title | Purification of replicating pancreatic β-cells for gene expression studies | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 675576 | - |
dc.date.updated | 2018-07-17T10:23:02Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 29235543 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB)) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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675576.pdf | Original Article | 3.45 MB | Adobe PDF | View/Open |
675576_Corr.pdf | Publisher Correction | 688.59 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License