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http://hdl.handle.net/2445/123874
Title: | An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays |
Author: | Höftberger, Romana Sabater, Lidia Marignier, Romain Aboul-Enein, Fahmy Bernard-Valnet, Raphaël Rauschka, Helmut Ruiz, Anne Blanco Morgado, Yolanda Graus Ribas, Francesc Dalmau Obrador, Josep Saiz Hinarejos, Albert |
Keywords: | Immunohistoquímica Immunoglobulines Immunohistochemistry Immunoglobulins |
Issue Date: | 4-Nov-2013 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0079083 |
It is part of: | PLoS One, 2013, vol. 8, num. 11, p. 1-6 |
URI: | http://hdl.handle.net/2445/123874 |
Related resource: | https://doi.org/10.1371/journal.pone.0079083 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Medicina) |
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