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Title: | Virulence genes and subclone status as markers of experimental virulence in a murine sepsis model among Escherichia coli sequence type 131 clinical isolates from Spain |
Author: | Merino, Irene Porter, Stephen B. Johnston, Brian D. Clabots, Connie Shaw Perujo, Evelyn Horcajada Gallego, Juan Pablo Cantón, Rafael Ruiz Garbajosa, Patricia Johnson, James R. |
Keywords: | Septicèmia Escheríchia coli Septicemia Escherichia coli |
Issue Date: | 30-Nov-2017 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Objective: To assess experimental virulence among sequence type 131 (ST131) Escherichia coli bloodstream isolates in relation to virulence genotype and subclone. Methods: We analysed 48 Spanish ST131 bloodstream isolates (2010) by PCR for ST131 subclone status (H30Rx, H30 non-Rx, or non-H30), virulence genes (VGs), and O-type. Then we compared these traits with virulence in a murine sepsis model, as measured by illness severity score (ISS) and rapid lethality (mean ISS >= 4). Results: Of the 48 study isolates, 65% were H30Rx, 21% H30 non-Rx, and 15% non-H30; 44% produced ESBLs, 98% were O25b, and 83% qualified as extraintestinal pathogenic E. coli (ExPEC). Of 49 VGs, ibeA and iss were associated significantly with non-H30 isolates, and sat, iha and malX with H30 isolates. Median VG scores differed by subclone, i.e., 12 (H30Rx), 10 (H30 non-Rx), and 11 (non-H30) (p < 0.01). Nearly 80% of isolates represented a described virotype. In mice, H30Rx and non-H30 isolates were more virulent than H30 non-Rx isolates (according to ISS [p = 0.03] and rapid lethality [p = 0.03]), as were ExPEC isolates compared with non-ExPEC isolates (median ISS, 4.3 vs. 2.7: p = 0.03). In contrast, most individual VGs, VG scores, VG profiles, and virotypes were not associated with mouse virulence. Conclusions: ST131 subclone and ExPEC status, but not individual VGs, VG scores or profiles, or virotypes, predicted mouse virulence. Given the lower virulence of non-Rx H30 isolates, hyper-virulence probably cannot explain the ST131-H30 clade's epidemic emergence. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0188838 |
It is part of: | PLoS One, 2017, vol. 12, num. 11,p. e0188838 |
URI: | http://hdl.handle.net/2445/124038 |
Related resource: | http://dx.doi.org/10.1371/journal.pone.0188838 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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MerinoI.pdf | 3.4 MB | Adobe PDF | View/Open |
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