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http://hdl.handle.net/2445/124165
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DC Field | Value | Language |
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dc.contributor.author | Oleksiewicz, Urszula | - |
dc.contributor.author | Gladych, Marta | - |
dc.contributor.author | Raman, Ayush T. | - |
dc.contributor.author | Heyn, Holger | - |
dc.contributor.author | Mereu, Elisabetta | - |
dc.contributor.author | Chlebanowska, Paula | - |
dc.contributor.author | Andrzejewska, Anastazja | - |
dc.contributor.author | Sozanska, Barbara | - |
dc.contributor.author | Samant, Neha | - |
dc.contributor.author | Fak, Katarzyna | - |
dc.contributor.author | Auguscik, Paulina | - |
dc.contributor.author | Kosinski, Marcin | - |
dc.contributor.author | Wroblewska, Joanna P. | - |
dc.contributor.author | Tomczak, Katarzyna | - |
dc.contributor.author | Kulcenty, Katarzyna | - |
dc.contributor.author | Ploski, Rafal | - |
dc.contributor.author | Biecek, Przemyslaw | - |
dc.contributor.author | Esteller, Manel | - |
dc.contributor.author | Shah, Parantu K. | - |
dc.contributor.author | Rai, Kunal | - |
dc.contributor.author | Wiznerowicz, Maciej | - |
dc.date.accessioned | 2018-08-29T09:31:08Z | - |
dc.date.available | 2018-08-29T09:31:08Z | - |
dc.date.issued | 2017-12-01 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2445/124165 | - |
dc.description.abstract | Reprogramming to induced pluripotent stem cells (iPSCs) and differentiation of pluripotent stem cells (PSCs) are regulated by epigenetic machinery. Tripartite motif protein 28 (TRIM28), a universal mediator of Kruppel-associated box domain zinc fingers (KRAB-ZNFs), is known to regulate both processes; however, the exact mechanism and identity of participating KRAB-ZNF genes remain unknown. Here, using a reporter system, we show that TRIM28/KRAB-ZNFs alter DNA methylation patterns in addition to H3K9me3 to cause stable gene repression during reprogramming. Using several expression datasets, we identified KRAB-ZNFs (ZNF114, ZNF483, ZNF589) in the human genome that maintain pluripotency. Moreover, we identified target genes repressed by these KRAB-ZNFs. Mechanistically, we demonstrated that these KRAB-ZNFs directly alter gene expression of important developmental genes by modulating H3K9me3 and DNA methylation of their promoters. In summary, TRIM28 employs KRAB-ZNFs to evoke epigenetic silencing of its target differentiation genes via H3K9me3 and DNA methylation. | - |
dc.format.extent | 16 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.stemcr.2017.10.031 | - |
dc.relation.ispartof | Stem Cell Reports, 2017, vol. 9, num. 6, p. 2065-2080 | - |
dc.relation.uri | https://doi.org/10.1016/j.stemcr.2017.10.031 | - |
dc.rights | cc-by (c) Oleksiewicz, Urszula et al., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Cèl·lules mare | - |
dc.subject.classification | Epigenètica | - |
dc.subject.classification | ADN | - |
dc.subject.other | Stem cells | - |
dc.subject.other | Epigenetics | - |
dc.subject.other | DNA | - |
dc.title | TRIM28 and Interacting KRAB-ZNFs Control Self-Renewal of Human Pluripotent Stem Cells through Epigenetic Repression of Pro-differentiation Genes | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 676878 | - |
dc.date.updated | 2018-08-29T09:31:09Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 29198826 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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676878.pdf | 4.97 MB | Adobe PDF | View/Open |
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