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DC Field | Value | Language |
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dc.contributor.author | Breen, Michael P., 1940- | - |
dc.contributor.author | Camps Camprubí, Marta | - |
dc.contributor.author | Carvalho-Simoes, Francisco | - |
dc.contributor.author | Zorzano Olarte, Antonio | - |
dc.contributor.author | Pilch, Paul F. | - |
dc.date.accessioned | 2018-09-17T13:11:26Z | - |
dc.date.available | 2018-09-17T13:11:26Z | - |
dc.date.issued | 2012-04-06 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/124628 | - |
dc.description.abstract | Caveolae, little caves of cell surfaces, are enriched in cholesterol, a certain level of which is required for their structural integrity. Here we show in adipocytes that cavin-2, a peripheral membrane protein and one of 3 cavin isoforms present in caveolae from non-muscle tissue, is degraded upon cholesterol depletion in a rapid fashion resulting in collapse of caveolae. We exposed 3T3-L1 adipocytes to the cholesterol depleting agent methyl-β-cyclodextrin, which results in a sudden and extensive degradation of cavin-2 by the proteasome and a concomitant movement of cavin-1 from the plasma membrane to the cytosol along with loss of caveolae. The recovery of cavin-2 at the plasma membrane is cholesterol-dependent and is required for the return of cavin-1 from the cytosol to the cell surface and caveolae restoration. Expression of shRNA directed against cavin-2 also results in a cytosolic distribution of cavin-1 and loss of caveolae. Taken together, these data demonstrate that cavin-2 functions as a cholesterol responsive component of caveolae that is required for cavin-1 localization to the plasma membrane, and caveolae structural integrity. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0034516 | - |
dc.relation.ispartof | PLoS One, 2012, vol. 7, num. 4, p. 1-8 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0034516 | - |
dc.rights | cc-by (c) Breen, Michael P., 1940- et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Colesterol | - |
dc.subject.classification | Proteïnes de membrana | - |
dc.subject.classification | Cèl·lules animals | - |
dc.subject.other | Cholesterol | - |
dc.subject.other | Membrane proteins | - |
dc.subject.other | Animal cells | - |
dc.title | Cholesterol depletion in adipocytes causes caveolae collapse concomitant with proteosomal degradation of cavin-2 in a switch-like fashion | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 639335 | - |
dc.date.updated | 2018-09-17T13:11:26Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22493697 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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File | Description | Size | Format | |
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639335.pdf | 1.64 MB | Adobe PDF | View/Open |
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