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http://hdl.handle.net/2445/125274
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DC Field | Value | Language |
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dc.contributor.author | Pérez-Lago, Laura | - |
dc.contributor.author | Izco, Santiago | - |
dc.contributor.author | Herranz, Marta | - |
dc.contributor.author | Tudó i Vilanova, Griselda | - |
dc.contributor.author | Carcelén, María | - |
dc.contributor.author | Comas, Iñaki | - |
dc.contributor.author | Sierra, Olalla | - |
dc.contributor.author | González Martín, Julián | - |
dc.contributor.author | Ruiz-Serrano, María J. | - |
dc.contributor.author | Eyene, Juan | - |
dc.contributor.author | Bouza, Emilio | - |
dc.contributor.author | García de Viedma, Darío | - |
dc.date.accessioned | 2018-10-10T15:43:59Z | - |
dc.date.available | 2018-10-10T15:43:59Z | - |
dc.date.issued | 2016-10-13 | - |
dc.identifier.issn | 1198-743X | - |
dc.identifier.uri | http://hdl.handle.net/2445/125274 | - |
dc.description.abstract | OBJECTIVE: Molecular epidemiology techniques in tuberculosis (TB) can identify high-risk strains that are actively transmitted. We aimed to implement a novel strategy to optimize the identification and control of multidrug-resistant (MDR) TB in a specific population. METHODS: We developed a strain-specific PCR tailored from whole genome sequencing (WGS) data to track a specific MDR prevalent strain in Equatorial Guinea (EG-MDR). RESULTS: The PCR was applied prospectively on remnants of GeneXpert reaction mixtures owing to the lack of culture facilities in Equatorial Guinea. In 147 (93%) of 158 cases, we were able to differentiate between infection by the EG-MDR strain or by any other strain and found that 44% of all rifampicin-resistant TB cases were infected by EG-MDR. We also analysed 93 isolates obtained from Equatorial Guinea 15 years ago, before MDR-TB had become the problem it is today. We found that two of the scarce historical MDR cases were infected by EG-MDR. WGS revealed low variability-six single nucleotide polymorphisms acquired by this strain over 15 years-likely because of the lack in the country of a specific program to treat MDR-TB. CONCLUSIONS: Our novel strategy, which integrated WGS analysis and strain-specific PCRs, represents a low-cost, rapid and transferable strategy that allowed a prospective efficient survey and fast historical analysis of MDR-TB in a population. | - |
dc.format.extent | 21 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | European Society of Clinical Microbiology and Infectious Diseases | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.cmi.2016.10.006 | - |
dc.relation.ispartof | Clinical Microbiology and Infection, 2016, vol. 23, num. 2, p. 92-97 | - |
dc.relation.uri | https://doi.org/10.1016/j.cmi.2016.10.006 | - |
dc.rights | (c) Pérez-Lago, Laura et al., 2016 | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Genòmica | - |
dc.subject.classification | Mycobacterium tuberculosis | - |
dc.subject.classification | Guinea Equatorial | - |
dc.subject.other | Genomics | - |
dc.subject.other | Mycobacterium tuberculosis | - |
dc.subject.other | Equatorial Guinea | - |
dc.title | A novel strategy based on genomics and specific PCR reveals how a multidrug resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 664918 | - |
dc.date.updated | 2018-10-10T15:43:59Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/638553/EU//TB-ACCELERATE | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27746398 | - |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) |
Files in This Item:
File | Description | Size | Format | |
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664918.pdf | 135.88 kB | Adobe PDF | View/Open |
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