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http://hdl.handle.net/2445/125706
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DC Field | Value | Language |
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dc.contributor.author | Moshe Reuven, Eliran | - |
dc.contributor.author | Leviatan Ben-Arye, Shani | - |
dc.contributor.author | Marshanski, Tal | - |
dc.contributor.author | Breimer, Michael E. | - |
dc.contributor.author | Yu, Hai | - |
dc.contributor.author | Fellah-Hebia, Imen | - |
dc.contributor.author | Roussel, Jean-Christian | - |
dc.contributor.author | Costa Vallés, Cristina | - |
dc.contributor.author | Galinanes, Manuel | - |
dc.contributor.author | Máñez Mendiluce, Rafael | - |
dc.contributor.author | Tourneau, Thierry Le | - |
dc.contributor.author | Soulillou, Jean-Paul | - |
dc.contributor.author | Cozzi, Emanuele | - |
dc.contributor.author | Chen, Xi | - |
dc.contributor.author | Padler-Karavani, Vered | - |
dc.date.accessioned | 2018-10-29T14:30:39Z | - |
dc.date.available | 2018-10-29T14:30:39Z | - |
dc.date.issued | 2016-09-01 | - |
dc.identifier.uri | http://hdl.handle.net/2445/125706 | - |
dc.description.abstract | Background: The two common sialic acids (Sias) in mammals are N-acetylneuraminic acid (Neu5Ac) and its hydroxylated form N-glycolylneuraminic acid (Neu5Gc). Unlike most mammals, humans cannot synthesize Neu5Gc that is considered foreign and recognized by circulating antibodies. Thus, Neu5Gc is a potential xenogenic carbohydrate antigen in bioprosthetic heart valves (BHV) that tend to deteriorate in time within human patients. Methods: We investigated Neu5Gc expression in non-engineered animal-derived cardiac tissues and in clinically used commercial BHV, and evaluated Neu5Gc immunogenicity on BHV through recognition by human anti-Neu5Gc IgG. Results: Neu5Gc was detected by immunohistochemistry in porcine aortic valves and in porcine and bovine pericardium. Qualitative analysis of Sia linkages revealed Siaa2-3> Siaa2-6 on porcine/bovine pericardium while the opposite in porcine aortic/pulmonary valve cusps. Similarly, six commercial BHV containing either porcine aortic valve or porcine/bovine/equine pericardium revealed Siaa2-3> Siaa2-6 expression. Quantitative analysis of Sia by HPLC showed porcine/bovine pericardium express 4-fold higher Neu5Gc levels compared to the porcine aortic/pulmonary valves, with Neu5Ac at 6-fold over Neu5Gc. Likewise, Neu5Gc was expressed on commercial BHV (186.3 +/- 16.9 pmol Sia/mu g protein), with Neu5Ac at 8-fold over Neu5Gc. Affinity-purified human anti-Neu5Gc IgG showing high specificity toward Neu5Gc-glycans (with no binding to Neu5Ac-glycans) on a glycan microarray, strongly bound to all tested commercial BHV, demonstrating Neu5Gc immune recognition in cardiac xenografts. Conclusions: We conclusively demonstrated Neu5Gc expression in native cardiac tissues, as well as in six commercial BHV. These Neu5Gc xeno-antigens were recognized by human anti-Neu5Gc IgG, supporting their immunogenicity. Altogether, these findings suggest BHV-Neu5Gc/anti-Neu5Gc may play a role in valve deterioration warranting further investigation. | - |
dc.format.extent | 24 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1111/xen.12260 | - |
dc.relation.ispartof | Xenotransplantation, 2016, vol. 23, num. 5, p. 381-392 | - |
dc.relation.uri | https://doi.org/10.1111/xen.12260 | - |
dc.rights | (c) John Wiley and Sons, 2016 | - |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Trasplantament d'òrgans | - |
dc.subject.classification | Malalties del cor | - |
dc.subject.other | Transplantation of organs | - |
dc.subject.other | Heart diseases | - |
dc.title | Characterization of immunogenic Neu5Gc in bioprosthetic heart valves | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.date.updated | 2018-07-24T12:17:33Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/327726/EU//XENO-AUTOANTIBODIES | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/603049/EU//TRANSLINK | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27610947 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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ReuvenEM.pdf | 3.37 MB | Adobe PDF | View/Open |
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