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http://hdl.handle.net/2445/125782
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DC Field | Value | Language |
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dc.contributor.author | Deplus, Rachel | - |
dc.contributor.author | Blanchon, Loïc | - |
dc.contributor.author | Rajavelu, Arumugam | - |
dc.contributor.author | Boukaba, Abdelhalim | - |
dc.contributor.author | Defrance, Matthieu | - |
dc.contributor.author | Luciani, Judith | - |
dc.contributor.author | Rothé, Françoise | - |
dc.contributor.author | Dedeurwaerder, Sarah | - |
dc.contributor.author | Denis, Hélène | - |
dc.contributor.author | Brinkman, Arie B. | - |
dc.contributor.author | Simmer, Femke | - |
dc.contributor.author | Müller, Fabian | - |
dc.contributor.author | Bertin, Benjamin | - |
dc.contributor.author | Berdasco, María | - |
dc.contributor.author | Putmans, Pascale | - |
dc.contributor.author | Calonne, Emilie | - |
dc.contributor.author | Litchfield, David W. | - |
dc.contributor.author | Launoit, Yvan de | - |
dc.contributor.author | Jurkowski, Tomasz P. | - |
dc.contributor.author | Stunnenberg, Hendrik G. | - |
dc.contributor.author | Bock, Christoph | - |
dc.contributor.author | Sotiriou, Christos | - |
dc.contributor.author | Fraga, Mario F. | - |
dc.contributor.author | Esteller, Manel | - |
dc.contributor.author | Jeltsch, Albert | - |
dc.contributor.author | Fuks, François | - |
dc.date.accessioned | 2018-10-31T13:28:35Z | - |
dc.date.available | 2018-10-31T13:28:35Z | - |
dc.date.issued | 2014-08-07 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | http://hdl.handle.net/2445/125782 | - |
dc.description.abstract | DNA methylation is a central epigenetic modification that is established by de novo DNA methyltransferases. The mechanisms underlying the generation of genomic methylation patterns are still poorly understood. Using mass spectrometry and a phosphospecific Dnmt3a antibody, we demonstrate that CK2 phosphorylates endogenous Dnmt3a at two key residues located near its PWWP domain, thereby downregulating the ability of Dnmt3a to methylate DNA. Genome-wide DNA methylation analysis shows that CK2 primarily modulates CpG methylation of several repeats, most notably of Alu SINEs. This modulation can be directly attributed to CK2-mediated phosphorylation of Dnmt3a. We also find that CK2-mediated phosphorylation is required for localization of Dnmt3a to heterochromatin. By revealing phosphorylation as a mode of regulation of de novo DNA methyltransferase function and by uncovering a mechanism for the regulation of methylation at repetitive elements, our results shed light on the origin of DNA methylation patterns. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.celrep.2014.06.048 | - |
dc.relation.ispartof | Cell Reports, 2014, vol. 8, p. 743-753 | - |
dc.relation.uri | https://doi.org/10.1016/j.celrep.2014.06.048 | - |
dc.rights | cc-by (c) Deplus, Rachel et al., 2014 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Fosforilació | - |
dc.subject.classification | Metilació | - |
dc.subject.classification | ADN | - |
dc.subject.classification | Epigenètica | - |
dc.subject.other | Phosphorylation | - |
dc.subject.other | Methylation | - |
dc.subject.other | DNA | - |
dc.subject.other | Epigenetics | - |
dc.title | Regulation of DNA Methylation Patterns by CK2-Mediated Phosphorylation of Dnmt3a | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 662683 | - |
dc.date.updated | 2018-10-31T13:28:35Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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