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Title: | Reference values assessment in a Mediterranean population for small dense low-density lipoprotein concentration isolated by an optimized precipitation method |
Author: | Fernández Cidón, Bárbara Padró i Miquel, Ariadna Alía Ramos, Pedro Castro Castro, María José Fanlo Maresma, Marta Dot Bach, Dolors Valero Politi, José Pintó Sala, Xavier Candás Estébanez, Beatriz |
Keywords: | Colesterol Avaluació del risc per la salut Malalties cardiovasculars Cholesterol Health risk assessment Cardiovascular diseases |
Issue Date: | 6-Jun-2017 |
Publisher: | Dove Medical Press |
Abstract: | Background: High serum concentrations of small dense low-density lipoprotein cholesterol (sd-LDL-c) particles are associated with risk of cardiovascular disease (CVD). Their clinical application has been hindered as a consequence of the laborious current method used for their quantification. Objective: Optimize a simple and fast precipitation method to isolate sd-LDL particles and establish a reference interval in a Mediterranean population. Materials and methods: Forty-five serum samples were collected, and sd-LDL particles were isolated using a modified heparin-Mg2+ precipitation method. sd-LDL-c concentration was calculated by subtracting high-density lipoprotein cholesterol (HDL-c) from the total cholesterol measured in the supernatant. This method was compared with the reference method (ultracentrifugation). Reference values were estimated according to the Clinical and Laboratory Standards Institute and The International Federation of Clinical Chemistry and Laboratory Medicine recommendations. sd-LDL-c concentration was measured in serums from 79 subjects with no lipid metabolism abnormalities. Results: The Passing-Bablok regression equation is y = 1.52 (0.72 to 1.73) + 0.07x (−0.1 to 0.13), demonstrating no significant statistical differences between the modified precipitation method and the ultracentrifugation reference method. Similarly, no differences were detected when considering only sd-LDL-c from dyslipidemic patients, since the modifications added to the precipitation method facilitated the proper sedimentation of triglycerides and other lipoproteins. The reference interval for sd-LDL-c concentration estimated in a Mediterranean population was 0.04-0.47 mmol/L. Conclusion: An optimization of the heparin-Mg2+ precipitation method for sd-LDL particle isolation was performed, and reference intervals were established in a Spanish Mediterranean population. Measured values were equivalent to those obtained with the reference method, assuring its clinical application when tested in both normolipidemic and dyslipidemic subjects. |
Note: | Reproducció del document publicat a: https://doi.org/10.2147/VHRM.S132475 |
It is part of: | Vascular Health and Risk Management, 2017, vol. 13, p. 201-207 |
URI: | http://hdl.handle.net/2445/125868 |
Related resource: | https://doi.org/10.2147/VHRM.S132475 |
ISSN: | 1176-6344 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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