Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/126223
Title: | Quantification of interacting cognate odorants with olfactory receptors in nanovesicles |
Author: | Sanmartí Espinal, Marta Iavicoli, Patrizia Calò, Annalisa Taulés i Marín, Marta Galve Bosch, Roger Marco Colás, Ma. Pilar Samitier i Martí, Josep |
Keywords: | Olfacte Biosensors Olors Bioenginyeria Smell Biosensors Odors Bioengineering |
Issue Date: | 13-Dec-2017 |
Publisher: | Nature Publishing Group |
Abstract: | This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-017-16997-9 |
It is part of: | Scientific Reports, 2017, vol. 7, p. 17483 |
URI: | http://hdl.handle.net/2445/126223 |
Related resource: | https://doi.org/10.1038/s41598-017-16997-9 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) |
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